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Literature summary for 2.4.1.13 extracted from
- Cucumber fruit sucrose synthetase isozymes (1982), Phytochemistry, 21, 1241-1244.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| UDP | strong | Cucumis sativus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | 0.38 | UDPglucose | isozyme SS2 | Cucumis sativus | |
| 5.3 | 6 | UDPglucose | cosubstrate UDPfructose, isozyme SS2 | Cucumis sativus | |
| 11.5 | - |
D-fructose | isozyme SS1 | Cucumis sativus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activation | Cucumis sativus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 540000 | - |
gel filtration | Cucumis sativus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucumis sativus | - |
cucumber, isoforms SSI, SSII | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isozymes: SS1 and SS2, partial | Cucumis sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Cucumis sativus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, isozyme SS1, 4 months, isozyme SS2, t1/2: 3 weeks | Cucumis sativus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + D-fructose | - |
Cucumis sativus | ADP + sucrose | - |
r | |
| UDP + sucrose | - |
Cucumis sativus | UDP-glucose + D-fructose | - |
? | |
| UDP-alpha-D-glucose + D-fructose | - |
Cucumis sativus | UDP + sucrose | - |
? | |
| UDPglucose + D-fructose | - |
Cucumis sativus | UDP + sucrose | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
sucrose cleavage | Cucumis sativus |
| 7.5 | - |
sucrose synthesis, isozyme SS2 | Cucumis sativus |
| 9 | - |
sucrose synthesis | Cucumis sativus |
| 9 | - |
isozyme SS | Cucumis sativus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | about half-maximal activity at pH 5.0 and 10.0, sucrose synthesis | Cucumis sativus |
| 5 | 8 | about half-maximal activity at pH 5.0 and 8.0, sucrose cleavage | Cucumis sativus |
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