General Stability | Organism |
---|---|
repeated freeze-thawing leads to slight decrease of activity, even in the presence of PMSF | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
52 | 290 | sucrose | - |
Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition | Zea mays |
87000 | - |
x * 87000, SDS-PAGE | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
maize, isozymes SS1, SS2, sh1/sh1- (Black Mexican Sweet) or sh1bz1-m4-genotype | - |
Purification (Comment) | Organism |
---|---|
2 isozymes: SS1 and SS2 | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Zea mays | - |
endosperm | - |
Zea mays | - |
seedling | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6 | - |
isozyme SS2 | Zea mays |
10 | - |
isozyme SS1 | Zea mays |
Storage Stability | Organism |
---|---|
-80°C, 20% glycerol, 0.1-1.0 mM DTT, stable | Zea mays |
4°C, prolonged storage leads to slight decrease of activity, even in the presence of PMSF | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP + sucrose | also substrate: ADP | Zea mays | UDP-glucose + D-fructose | - |
? | |
UDP + sucrose | substrate: TDP | Zea mays | UDP-glucose + D-fructose | - |
? | |
UDP + sucrose | with SS2-isozyme TDP is 57%, with SS1-isozyme 91% as effective as UDP | Zea mays | UDP-glucose + D-fructose | - |
? | |
UDP + sucrose | ADP is less effective | Zea mays | UDP-glucose + D-fructose | - |
? | |
UDPglucose + D-fructose | - |
Zea mays | UDP + sucrose | - |
r |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 87000, SDS-PAGE | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 5.8, isozyme SS1 | Zea mays |