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Literature summary for 2.4.1.13 extracted from
- Purification and properties of sucrose synthase from maize (1978), Plant Physiol., 61, 389-393.
General Stability
| General Stability | Organism |
|---|---|
| repeated freeze-thawing, stable to if foaming is avoided | Zea mays |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 3 mM | Zea mays |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation | Zea mays | |
| Mg2+ | sucrose synthesis, 0.1-10.0 mM | Zea mays | |
| Mn2+ | activation | Zea mays | |
| Mn2+ | inhibits at 3 mM | Zea mays | |
| Mn2+ | 0.1 mM | Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Zea mays |
| 88000 | - |
4 * 88000, SDS-PAGE, depending on the ionic species and ionic strength of the solution, the enzyme can aquire catalytically active, tetrameric, octameric and other higher aggregated forms of which the tetramer is the predominant form | Zea mays |
| 360000 | - |
analytical ultracentrifugation in the presence of Mg2+ | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.85 | - |
- |
Zea mays |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, about 75% of activity retained after 4 months | Zea mays |
| 4°C, storage leads to gradual precipitation of denatured protein | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP + sucrose | - |
Zea mays | UDP-glucose + D-fructose | - |
? | |
| UDPglucose + D-fructose | - |
Zea mays | UDP + sucrose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 88000, SDS-PAGE, depending on the ionic species and ionic strength of the solution, the enzyme can aquire catalytically active, tetrameric, octameric and other higher aggregated forms of which the tetramer is the predominant form | Zea mays |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
and above, 5 min, rapid loss of activity | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
sucrose cleavage | Zea mays |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
below, complete inactivation | Zea mays |
| 5.5 | 8 | stable | Zea mays |
| 8 | - |
above, gradual loss of activity | Zea mays |
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Release 2023.1 (January 2023)
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