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Literature summary for 2.4.1.123 extracted from

  • Wang, Z.; Zhu, Y.; Wang, L.; Liu, X.; Liu, Y.; Phillips, J.; Deng, X.
    A WRKY transcription factor participates in dehydration tolerance in Boea hygrometrica by binding to the W-box elements of the galactinol synthase (BhGolS1) promoter (2009), Planta, 230, 1155-1166.
    View publication on PubMed
Search for more literature for 2.4.1.123

Cloned(Commentary)

Cloned (Comment) Organism
coding region of BhGolS1 is inframe cloned into pGEX-4T-1 downstream to GST coding region and transformed into Escherichia coli BL21(DE3) and BL21 codon plus cells, generation of transgenic tobacco plants, constructs are introduced into Agrobacterium strain LBA4404 by electroporation and transformed into tobacco via a leaf disc method Dorcoceras hygrometricum

Metals/Ions

Metals/Ions Comment Organism Structure
MnCl2 5 mM are included in assay medium Dorcoceras hygrometricum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-galactose + myo-inositol Dorcoceras hygrometricum
-
 UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol i.e. galactinol ?

Organism

Organism UniProt Comment Textmining
Dorcoceras hygrometricum B6UYJ2
-
-

Purification (Commentary)

Purification (Comment) Organism
the soluble protein is separated by SDS-PAGE to check the molecular weight of the recombinant protein, which is then purified using Glutathione Sepharose TM 4B Dorcoceras hygrometricum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Dorcoceras hygrometricum
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seedling
-
 Dorcoceras hygrometricum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 BhGolS1 activity enhances drought tolerance in transgenic tobacco, a higher survival rate of transgenic plants after rewatering (84.8% for S10-1 tobacco line, 72.6% for S22-5 tobacco line, 61.5% for Rd15-6 tobacco line), compared to wild type (38.4%) is observed Dorcoceras hygrometricum
3.8
-
 purified BhGolS1-GST fusion protein is able to catalyze galactinol synthesis, 50 mM Hepes buffer, pH 7.0, 2 mM dithiothreitol, 5 mM MnCl2 and 0.5-1 mg purified enzyme protein Dorcoceras hygrometricum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + myo-inositol
-
 Dorcoceras hygrometricum UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol i.e. galactinol ?

Synonyms

Synonyms Comment Organism
BhGolS1
-
 Dorcoceras hygrometricum
galactinol synthase
-
 Dorcoceras hygrometricum
GOLS
-
 Dorcoceras hygrometricum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Dorcoceras hygrometricum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Dorcoceras hygrometricum

Expression

Organism Comment Expression
Dorcoceras hygrometricum a WRKY transcription factor participates in dehydration tolerance in Boea hygrometrica by binding to the W-box elements of the galactinol synthase promoter, GolS genes are induced by a variety of stresses in both stress-sensitive and tolerant-plant species, mRNA and protein accumulate in leaves dehydrated for 2-48 h, and disappear after rehydration, BhGolS1 is rapidly induced by abscisic acid at the mRNA level after 0.5 h and at the protein level after 8 h up

General Information

General Information Comment Organism
physiological function first committed step of the raffinose family oligosaccharides biosynthetic pathway Dorcoceras hygrometricum