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Literature summary for 2.4.1.123 extracted from

  • Sprenger, N.; Keller, F.
    Allocation of raffinose family oligosaccharides to transport and storage pools in Ajuga reptans: the roles of two distinct galactinol synthases (2000), Plant J., 21, 249-258.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information cold-inducible GolS-1 and 2 genes Ajuga reptans

Cloned(Commentary)

Cloned (Comment) Organism
cold-inducible GolS-1 and -2 genes encode 2 distinct galactinol synthases, cloning and sequencing of the GolS-1 and -2 genes, deduced amino acid sequences, expression of GolS-1 cDNA in Escherichia coli as functional enzyme Ajuga reptans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-galactose + myo-inositol Ajuga reptans galactinol synthesis, which is the galactosyl donor for the synthesis of raffinose and stachyose, at least 2 isoforms: GolS-1 is mainly involved in the synthesis of storage raffinose family oligosaccharides and GolS-2 in the synthesis of transport raffinose family oligosaccharides, GolS-2 expression is much lower than that of GolS-1, galactinol may be catabolized by the reverse reaction UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
?
UDP-galactose + myo-inositol Ajuga reptans first committed enzyme in biosynthesis of raffinose family oligosaccharides, which play a role in plant stress tolerance UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
?

Organism

Organism UniProt Comment Textmining
Ajuga reptans Q9XGN3 GolS-2, 2 isoforms: GolS-1 and GolS-2
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Ajuga reptans Q9XGN4 GolS-1, 2 isoforms: GolS-1 and GolS-2
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Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein putative serine phosphorylation site at GolS-1 codon position 263 Ajuga reptans

Source Tissue

Source Tissue Comment Organism Textmining
leaf source and sink leaves, GolS-1 is source leaf-specific, equally weak expression of GolS-2 in all leaf types Ajuga reptans
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mesophyll GolS-1 is primarily expressed in the mesophyll, the site of raffinose family oligosaccharides storage Ajuga reptans
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phloem GolS-2 is primarily expressed in the phloem-associated intermediary cells known for their role in raffinose family oligosaccharides phloem loading Ajuga reptans
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + myo-inositol reaction is probably reversible Ajuga reptans UDP + 1-O-alpha-D-galactosyl-D-myo-inositol identical with galactinol r
UDP-galactose + myo-inositol galactinol synthesis, which is the galactosyl donor for the synthesis of raffinose and stachyose, at least 2 isoforms: GolS-1 is mainly involved in the synthesis of storage raffinose family oligosaccharides and GolS-2 in the synthesis of transport raffinose family oligosaccharides, GolS-2 expression is much lower than that of GolS-1, galactinol may be catabolized by the reverse reaction Ajuga reptans UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
?
UDP-galactose + myo-inositol first committed enzyme in biosynthesis of raffinose family oligosaccharides, which play a role in plant stress tolerance Ajuga reptans UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Ajuga reptans