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Literature summary for 2.4.1.123 extracted from

  • Liu, J.J.; Odegard, W.; de Lumen, B.O.
    Galactinol synthase from kidney bean cotyledon and zucchini leaf. Purification and N-terminal sequences (1995), Plant Physiol., 109, 505-511.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.123

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol absolute requirement Phaseolus vulgaris

General Stability

General Stability Organism
ammonium sulfate fractionation causes 32% loss of enzyme activity Phaseolus vulgaris
bovine serum albumin is essential to preserve enzyme activity during the assay Phaseolus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 UDP-galactose
-
 Phaseolus vulgaris
4.5
-
 myo-inositol
-
 Phaseolus vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ absolute requirement Phaseolus vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 x * 36000, SDS-PAGE Cucurbita pepo
38000
-
 x * 38000, SDS-PAGE Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-galactose + myo-inositol Cucurbita pepo
-
 UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
 ?
UDP-galactose + myo-inositol Phaseolus vulgaris key enzyme in controlling galactose oligosaccharide biosynthesis, which plays a role in seed desiccation tolerance UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
 ?

Organism

Organism UniProt Comment Textmining
Cucurbita pepo
-
 var. Black Beauty
-
Phaseolus vulgaris
-
 var. Big Red
-

Purification (Commentary)

Purification (Comment) Organism
1591fold, copurified with a 41 and 43 kDa peptide Phaseolus vulgaris
2472fold Cucurbita pepo

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon highest activity among all of the components of seed, much poorer source than Cucurbita pepo mature leaves Phaseolus vulgaris
-
leaf mature leaves, much richer source than Phaseolus vulgaris cotyledons Cucurbita pepo
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.75
-
-
 Phaseolus vulgaris
32.1
-
-
 Cucurbita pepo

Storage Stability

Storage Stability Organism
-20°C, enzyme in ammonium sulfate precipitate form, 6-8 weeks, stable Phaseolus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + myo-inositol
-
 Cucurbita pepo UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
 ?
UDP-galactose + myo-inositol
-
 Cucurbita pepo UDP + 1-O-alpha-D-galactosyl-D-myo-inositol identical with galactinol ?
UDP-galactose + myo-inositol high specific affinity for UDP-Gal and myo-inositol Phaseolus vulgaris UDP + 1-O-alpha-D-galactosyl-D-myo-inositol identical with galactinol ?
UDP-galactose + myo-inositol key enzyme in controlling galactose oligosaccharide biosynthesis, which plays a role in seed desiccation tolerance Phaseolus vulgaris UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Phaseolus vulgaris
? x * 36000, SDS-PAGE Cucurbita pepo

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Phaseolus vulgaris
30
-
 assay at Cucurbita pepo

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Phaseolus vulgaris