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Literature summary for 2.4.1.122 extracted from
- Co-purification of galactosyltransferases from chick-embryo liver (1985), Biochem. J., 227, 573-582.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-lactalbumin | - |
Gallus gallus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
UDP-galactose | - |
Gallus gallus |  |
| 5 | - |
asialo ovine submaxillary mucin | in terms of N-acetylgalactosamine equivalents | Gallus gallus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Gallus gallus | 16020 | - |
| microsome | - |
Gallus gallus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activation, 5-10 mM | Gallus gallus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
x * 68000, SDS-PAGE | Gallus gallus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| solubilized with 1% Triton X-100 in 0.1 M NaCl | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Gallus gallus | - |
| liver | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Gallus gallus |
| 0.00428 | - |
purified enzyme | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | poor substrates are low-molecular weight acceptors, e.g. N-acetylgalactosamine | Gallus gallus | ? | - |
? | |
| UDP-galactose + asialo ovine submaxillary mucin | best substrate | Gallus gallus | UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-asialo ovine submaxillary mucin | - |
? | |
| UDP-galactose + asialo-agalacto-alpha1-acid glycoprotein | - |
Gallus gallus | UDP + galactosyl-beta-1,3-asialo-agalacto-alpha1-acid glycoprotein | - |
? | |
| UDP-galactose + ganglioside GM2 | - |
Gallus gallus | UDP + galactosyl-beta-1,3-ganglioside GM2 | - |
? | |
| UDP-galactose + glycoprotein N-acetyl-D-galactosamine | - |
Gallus gallus | UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine | - |
? | |
| UDP-galactose + N-acetyl-D-galactosamine | - |
Gallus gallus | UDP + beta-D-galactosyl-1,3-N-acetyl-D-galactosamine | - |
? | |
| UDP-galactose + N-acetyl-D-glucosamine | - |
Gallus gallus | UDP + beta-D-galactosyl-1,3-N-acetyl-D-glucosamine | - |
? | |
| UDP-galactose + N-acetylgalactosaminide mucin | - |
Gallus gallus | UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 68000, SDS-PAGE | Gallus gallus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 6.40 | Gallus gallus |
| 5 | 7 | broad | Gallus gallus |
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Release 2023.1 (January 2023)
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