Literature summary for 2.4.1.120 extracted from

Mittasch, J.; Mikolajewski, S.; Breuer, F.; Strack, D.; Milkowski, C.
Genomic microstructure and differential expression of the genes encoding UDP-glucose:sinapate glucosyltransferase (UGT84A9) in oilseed rape (Brassica napus) (2010), Theor. Appl. Genet., 120, 1485-1500.

Search for more literature for 2.4.1.120

Application

Application	Comment	Organism
agriculture	the genes encoding this enzyme are considered as potential targets for molecular breeding of low sinapine oilseed rape	Brassica napus var. napus

Cloned(Commentary)

Cloned (Comment)	Organism
heterologous expression in Escherichia coli strain BL21	Brassica napus var. napus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Brassica napus var. napus	5737	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-alpha-D-glucose + sinapate	Brassica napus var. napus	-	UDP + 1-O-sinapoyl-beta-D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Brassica napus var. napus	C1L318	UGT84A9a, gene locus belongs to sequence type 1, genome harbours 4 distinct gene loci; cv. Express	-
Brassica napus var. napus	C1L319	UGT84A9b, gene locus belongs to sequence type 1, genome harbours 4 distinct gene loci; cv. Express	-
Brassica napus var. napus	C1L320	UGT84A9c, gene locus belongs to sequence type 2, genome harbours 4 distinct gene loci; cv. Express	-
Brassica napus var. napus	C1L321	UGT84A9d, gene locus belongs to sequence type 2, genome harbours 4 distinct gene loci; cv. Express	-
Brassica napus var. napus	Q9FYU7	UGT84A9-1, sequence type 1; cv. Express	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
flower	-	Brassica napus var. napus	-
seed	at cotyledon stage and at well-developed mature embryo stage	Brassica napus var. napus	-
seedling	2-day-old	Brassica napus var. napus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-alpha-D-glucose + sinapate	-	Brassica napus var. napus	UDP + 1-O-sinapoyl-beta-D-glucose	-	?

Synonyms

Synonyms	Comment	Organism
SGT	-	Brassica napus var. napus
UDP-glucose:sinapate glucosyltransferase	-	Brassica napus var. napus
UGT84A9	-	Brassica napus var. napus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Brassica napus var. napus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	Brassica napus var. napus

Expression

Organism	Comment	Expression
Brassica napus var. napus	the genomic microstructures reveal the presence of several transposon-derived sequence elements at all 4 UGT84A9 loci which underlie increased reorganization, weakly expressed sequence type 2 orthologs UGT84A9c and d in the Brassica genomes might indicate that these gene copies are necessary for genetic robustness	additional information
Brassica napus var. napus	Brassicus napus harbors 2 distinguishable sequence types of the UGT84A9 gene designated as UGT84A9-1 and UGT84A9-2, UGT84A9-1 is the predominantly expressed variant, which is significantly up-regulated during the seed filling phase, when sinapate ester biosynthesis exhibits strongest activity	up

General Information

General Information	Comment	Organism
physiological function	key enzyme for sinapate ester biosynthesis, the glucosyltransferase UGT84A9 catalyzes the formation of 1-O-sinapoyl-D-glucose, which feeds as acyl donor into a broad range of accumulating sinapate esters, including the major antinutritive seed component sinapoylcholine (sinapine)	Brassica napus var. napus
physiological function	key enzyme for sinapate ester biosynthesis, the glucosyltransferase UGT84A9 catalyzes the formation of 1-O-sinapoyl-D-glucose, which feeds as acyl donor into a broad range of accumulating sinapate esters, including the major antinutritive seed component sinapoylcholine (sinapine)	Brassica napus var. napus

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Release 2023.1 (January 2023)