Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.120 extracted from

  • Wang, S.X.; Ellis, B.E.
    Enzymology of UDP-glucose:sinapic acid glucosyltransferase from Brassica napus (1998), Phytochemistry, 49, 307-318.
No PubMed abstract available
Search for more literature for 2.4.1.120

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Brassica napus
Hg2+
-
 Brassica napus
p-hydroxymercuribenzoate
-
 Brassica napus
TDP
-
 Brassica napus
UDP
-
 Brassica napus
UDPmannose weak inhibition Brassica napus
UDPxylose weak inhibition Brassica napus
Zn2+
-
 Brassica napus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 Sinapic acid
-
 Brassica napus
2.4
-
 UDPglucose
-
 Brassica napus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Brassica napus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ modest stimulation Brassica napus
Mn2+ modest stimulation Brassica napus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 gel filtration Brassica napus

Organism

Organism UniProt Comment Textmining
Brassica napus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brassica napus

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucose + sinapate = UDP + 1-O-sinapoyl-beta-D-glucose mechanism fits a random bi bi model Brassica napus
a

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Brassica napus
-

Storage Stability

Storage Stability Organism
-20°C, litte loss of activity after 1 month Brassica napus
4°C, less than 10% loss of activity after 2 d, more than 90% loss of activity after 1 month Brassica napus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-alpha-D-glucose + sinapic acid 96% of the activity with UDPglucose Brassica napus dTDP + 1-O-sinapoyl-beta-D-glucose
-
 r
UDP-alpha-D-glucose + 4-coumaric acid 21% of the activity with sinapic acid Brassica napus UDP + 1-O-(4-coumaroyl)-beta-D-glucose
-
 ?
UDP-alpha-D-glucose + 5-hydroxyferulic acid 39% of the activity with sinapic acid Brassica napus UDP + 1 O-(5-hydroxyferuloyl)-beta-D-glucose
-
 ?
UDP-alpha-D-glucose + caffeic acid 14% of the activity with sinapic acid Brassica napus UDP + 1-O-caffeoyl-beta-D-glucose
-
 ?
UDP-alpha-D-glucose + cinnamic acid 24% of the activity with sinapic acid Brassica napus UDP + 1-O-cinnamoyl-beta-D-glucose
-
 ?
UDP-alpha-D-glucose + sinapic acid
-
 Brassica napus UDP + 1-O-sinapoyl-beta-D-glucose
-
 r
UDP-alpha-D-glucose + syringic acid 10% of the activity with sinapic acid Brassica napus UDP + 1-O-syringoyl-beta-D-glucose
-
 ?
UDPalpha-D-glucose + ferulic acid 77% of the activity with sinapid acid Brassica napus UDP + 1-O-feruloyl-beta-D-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
-
 Brassica napus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
32 50 32°C: maximal activity, 50°C: no activity above Brassica napus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 2 d, less than 10% loss of activity Brassica napus
25
-
 24 h more than 90% loss of activity Brassica napus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 MES buffer Brassica napus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5 no activity detected below pH 4.5 and above pH 8.5 Brassica napus