Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | stimulation | Raphanus sativus | |
dithiothreitol | 10 mM, 3fold stimulation | Raphanus sativus | |
additional information | SH-group required | Raphanus sativus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PCMB | 100% inhibition at 1 mM, activity can be restored up to 50% by addition of 10 mM dithiothreitol, 24% inhibition at 0.1 mM, activity can be completely restored by 10 mM dithiothreitol | Raphanus sativus | |
UDP | strong | Raphanus sativus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + sinapic acid | Raphanus sativus | detoxification of sinapic acid and activation of sinapic acid for the subsequent sinapoyltransferase reaction leading to sinapoylmalate | UDP + 1-O-sinapoyl-beta-D-glucose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Raphanus sativus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Raphanus sativus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + 3,5-dihydroxybenzoic acid | 5% of activity compared to sinapic acid | Raphanus sativus | UDP + 1-O-(3,5-dihydroxybenzoyl)-beta-D-glucose | - |
? | |
UDP-alpha-D-glucose + 4-coumaric acid | 40% of activity compared to sinapic acid | Raphanus sativus | UDP + 1-O-(4-coumaroyl)-beta-D-glucose | - |
? | |
UDP-alpha-D-glucose + 4-hydroxybenzoic acid | 31% of activity compared to sinapic acid | Raphanus sativus | UDP + 1-O-(4-hydroxybenzoyl)-beta-D-glucose | - |
? | |
UDP-alpha-D-glucose + anthranilic acid | 31% of activity compared to sinapic acid | Raphanus sativus | UDP + 1-O-anthraniloyl-beta-D-glucose | - |
? | |
UDP-alpha-D-glucose + benzoic acid | 39% of activity compared to sinapic acid | Raphanus sativus | UDP + 1-O-benzoyl-beta-D-glucose | - |
? | |
UDP-alpha-D-glucose + caffeic acid | 38% of activity compared to sinapic acid | Raphanus sativus | UDP + 1-O-caffeoyl-beta-D-glucose | - |
? | |
UDP-alpha-D-glucose + cinnamic acid | 16% of activity compared to sinapic acid | Raphanus sativus | UDP + 1-O-cinnamoyl-beta-D-glucose | - |
? | |
UDP-alpha-D-glucose + sinapic acid | - |
Raphanus sativus | UDP + 1-O-sinapoyl-beta-D-glucose | - |
r | |
UDP-alpha-D-glucose + sinapic acid | detoxification of sinapic acid and activation of sinapic acid for the subsequent sinapoyltransferase reaction leading to sinapoylmalate | Raphanus sativus | UDP + 1-O-sinapoyl-beta-D-glucose | - |
r | |
UDP-alpha-D-glucose + syringic acid | 35% of activity compared to sinapic acid | Raphanus sativus | UDP + 1-O-syringoyl-beta-D-glucose | - |
? | |
UDP-alpha-D-glucose + vanillic acid | 36% of activity compared to sinapic acid | Raphanus sativus | UDP + 1-O-vanilloyl-beta-D-glucose | - |
? | |
UDPalpha-D-glucose + ferulic acid | 38% of activity compared to sinapic acid | Raphanus sativus | UDP + 1-O-feruloyl-beta-D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Raphanus sativus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | 25°C: 50% of maximal activity, 45°C: 80% of maximal activity | Raphanus sativus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Raphanus sativus |