Literature summary for 2.4.1.114 extracted from
Kleinhofs, A.; Haskins, F.A.; Gorz, H.J.
trans-o-Hydroxycinnamic acid glucosylation in cell-free extracts of Meliotus alba (1967), Phytochemistry, 6, 1313-1318.
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
2-mercaptoethanol |
stimulation |
Melilotus albus |
|
cysteine |
stimulation |
Melilotus albus |
|
General Stability
General Stability |
Organism |
dialysis overnight inactivates |
Melilotus albus |
freezing results in a complete loss of activity |
Melilotus albus |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
Cu2+ |
1 mM, 63% inhibition |
Melilotus albus |
|
additional information |
not inhibitory: 4 mM EDTA or 0.8 mM 2-coumaric acid glucoside |
Melilotus albus |
|
Sodium citrate |
4 mM, 37% inhibition |
Melilotus albus |
|
Zn2+ |
1 mM, 77% inhibition |
Melilotus albus |
|
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
UDP-glucose + 2-hydroxycinnamate |
Melilotus albus |
involved in the biosynthesis of coumarin |
UDP + (2E)-3-[2-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Melilotus albus |
- |
cucubb genotype |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
partial |
Melilotus albus |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
leaf |
- |
Melilotus albus |
- |
Storage Stability
Storage Stability |
Organism |
4°C, complete loss of activity within 1-2 days |
Melilotus albus |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
UDP-glucose + 2-hydroxycinnamate |
- |
Melilotus albus |
UDP + (2E)-3-[2-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid |
- |
? |
|
UDP-glucose + 2-hydroxycinnamate |
involved in the biosynthesis of coumarin |
Melilotus albus |
UDP + (2E)-3-[2-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoic acid |
- |
? |
|
Temperature Stability [°C]
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
4 |
- |
1-2 d, complete loss of activity |
Melilotus albus |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
7.6 |
8.3 |
- |
Melilotus albus |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
6.5 |
8.8 |
25% of maximal activity at pH 6, 10% of maximal activity at pH 9 |
Melilotus albus |