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Literature summary for 2.4.1.109 extracted from
- An efficient assay for dolichyl phosphate-mannose: protein O-mannosyltransferase (2002), Anal. Biochem., 307, 273-279.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | 10 mM, complete inhibition | Saccharomyces cerevisiae |  |
| glycerol | at 50% inhibitory | Saccharomyces cerevisiae | |
| additional information | no inhibition by EDTA | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
Tyr-Asn-Pro-Thr-Ser-Val | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Saccharomyces cerevisiae | 16020 | - |
| spheroplast | - |
Saccharomyces cerevisiae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, 10 mM | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae BY4743 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay development on microtiter plates | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val | - |
? | |
| dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val | - |
Saccharomyces cerevisiae BY4743 | dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PMT | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Saccharomyces cerevisiae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
40% remaining activity at 0°C | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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