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Literature summary for 2.4.1.109 extracted from
- Mannosylphosphoryldolichol-mediated O-mannosylation of yeast glycoproteins: stereospecificity and recognition of the alpha-isoprene unit by a purified mannosyltransferase (1995), Arch. Biochem. Biophys., 316, 773-779.
General Stability
| General Stability | Organism |
|---|---|
| stable in solution with deoxycholate and CHAPS | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glycerol | at 20% inhibitory | Saccharomyces cerevisiae |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Saccharomyces cerevisiae | 16020 | - |
| microsome | - |
Saccharomyces cerevisiae | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dolichyl phosphate D-mannose + protein | Saccharomyces cerevisiae | - |
dolichyl phosphate + O-D-mannosylprotein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
gene PMT1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
| solubilization with 0.5% deoxycholate and 1.2% CHAPS | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0035 | - |
partially purified enzyme | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, partially purified enzyme, indefinitely stable | Saccharomyces cerevisiae |
| 23°C, partially purified enzyme, 2% glycerol, at least 12 h stable | Saccharomyces cerevisiae |
| 4-23°C, partially purified enzyme, stable for at least 1 week | Saccharomyces cerevisiae |
| 4°C, solubilized with 0.5% Triton X-100, loss of 80% activity within 5 days | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dolichyl phosphate D-mannose + biotin-Tyr-Ala-Thr-Ala-Val-NH2 | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosyl-N-biotinyl-Tyr-Ala-Thr-Ala-Val-NH2 | - |
? | |
| dolichyl phosphate D-mannose + protein | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? | |
| dolichyl phosphate D-mannose + protein | the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues | Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? | |
| dolichyl phosphate D-mannose + protein | preferred chain lengthin decreasing order: C100, C80, C55, C35 | Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? | |
| dolichyl phosphate D-mannose + protein | strictly stereospecific for the anomeric configuration of phosphoryl-linkage of the donor substrate, a saturated alpha-isoprene unit in the dolichyl moiety is required | Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? | |
| dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val-NH2 | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-alpha-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val-NH2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PMT | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.9 | - |
inactivation below pH 4.9 | Saccharomyces cerevisiae |
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