Activating Compound | Comment | Organism | Structure |
---|---|---|---|
deoxycholate | 0.025%, stimulates slightly | Saccharomyces cerevisiae | |
additional information | not affected by Triton X-100 up to 0.2% | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by Triton X-100 up to 0.2% | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Saccharomyces cerevisiae | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ or Mn2+ stimulates | Saccharomyces cerevisiae | |
Mg2+ | optimal stimulation at 7 mM | Saccharomyces cerevisiae | |
Mn2+ | Mg2+ or Mn2+ stimulates | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + protein | Saccharomyces cerevisiae | production of cell-wall mannoproteins | dolichyl phosphate + O-D-mannosylprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00057 | - |
partially purified enzyme | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + protein | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? | |
dolichyl phosphate D-mannose + protein | the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues | Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
ir | |
dolichyl phosphate D-mannose + protein | acceptor substrate: HCl-treated cell wall mannoprotein from Saccharomyces cerevisiae | Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
ir | |
dolichyl phosphate D-mannose + protein | production of cell-wall mannoproteins | Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.7 | - |
- |
Saccharomyces cerevisiae |