Protein Variants | Comment | Organism |
---|---|---|
C217S | Cys217 although not required for activity per se, represents the only thiol that causes enzyme inactivation when modified. KM-value for UDP-N-acetyl-D-glucosamine is about 5% higher than the wild-type value, the Km-value for Galbeta(1-3)GalNAcalpha-p-nitrophenol is about 20% higher than the wild-type value.Unlike the wild-type enzyme the mutant enzyme can be stored at 4°C for at least 3 months without any loss of activity. The mutant enzyme is insensitive to DTNB, iodoacetamide, NEM, HgCl2 and ZnCl2 | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the mutant enzyme C217S is insensitive to DTNB, iodoacetamide, NEM, HgCl2 and ZnCl2 | Mus musculus | |
UDP | competitive inhibition versus donor substrate, wild-type enzyme and mutant enzyme C217S | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
Galbeta(1-3)GalNAcalpha-p-nitrophenol | pH 6.7, 37°C, wild-type enzyme | Mus musculus | |
0.13 | - |
Galbeta(1-3)GalNAcalpha-p-nitrophenol | pH 6.7, 37°C, mutant enzyme C217S | Mus musculus | |
1.1 | - |
UDP-N-acetyl-D-glucosamine | pH 6.7, 37°C, wild-type enzyme | Mus musculus | |
1.15 | - |
UDP-N-acetyl-D-glucosamine | pH 6.7, 37°C, mutant enzyme C217S | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
inactivation during storage may be caused by air oxidation and points to a requirement for inmodified thiols in the core protein | Mus musculus |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | both Asn58 and Asn95 are glycosylated | Mus musculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.2 | - |
mutant enzyme C217S | Mus musculus |
9.2 | - |
wild-type enzyme | Mus musculus |
Storage Stability | Organism |
---|---|
mutant enzyme C217S can be stored at 4°C for at least 3 months without any loss of activity, wild-type enzyme loses about 95% of its activity after 4 weeks | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GalNAcalpha-p-nitrophenol | - |
Mus musculus | UDP + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | in absence of dithiothreitol, one of the two free Cys residues (Cys217 and Cys235) is found to be engaged in an intermolecular bridge causing dimerization of the protein | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
core 2 beta1,6-N-acetylglucosaminyltransferase I | - |
Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
UDP | wild-type enzyme | Mus musculus | |
0.15 | - |
UDP | mutant enzyme C217S | Mus musculus |