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Literature summary for 2.4.1.102 extracted from

  • Yang, X.; Qin, W.; Lehotay, M.; Toki, D.; Dennis, P.; Schutzbach, J.S.; Brockhausen, I.
    Soluble human core 2 beta6-N-acetylglucosaminyltransferase C2GnT1 requires its conserved cysteine residues for full activity (2003), Biochim. Biophys. Acta, 1648, 62-74.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol stimulates up to 20fold Homo sapiens
dithiothreitol stimulates up to 20fold Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of the soluble catalytic domain in insect cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C100S mutant enzyme with 10% of wild-type activity Homo sapiens
C151S inactive mutant protein Homo sapiens
C172S inactive mutant protein Homo sapiens
C199S inactive mutant protein Homo sapiens
C217S mutant enzyme with 41% of wild-type activity. Similar Km and Vmax values for Galbeta(1-3)GalNAcalpha-p-nitrophenol, Km-value for UDP-N-acetyl-D-glucosamine is 2.9fold higher than wild-type value. In contrast to wild-type enzyme, the mutant enzyme is not inhibited by sulfhydryl reagents and is not stimulated by reducing agents Homo sapiens
C372S inactive mutant protein Homo sapiens
C381S inactive mutant protein Homo sapiens
C413S inactive mutant protein Homo sapiens
C59S inactive mutant protein Homo sapiens
DELTA407-428 inactive mutant protein Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis-2-nitrobenzoic acid inactivation is partially prevented by prior addition of donor or acceptor substrate and by sulfhydryl reducing agents. 1 mM inhibits 94% Homo sapiens
diethyldicarbonate 0.2 mM, 74% inhibition Homo sapiens
Hydroxyphenylglyoxal 19% inhibition at 0.25 mM, complete inhibition at 5-10 mM Homo sapiens
iodoacetate 0.2 mM inhibits the enzyme 70% Homo sapiens
additional information enzyme is fully active in presence of 5 mM EDTA Homo sapiens
N-bromosuccinimide 5 mM, complete inhibition Homo sapiens
NEM inactivation is partially prevented by prior addition of donor or acceptor substrate and by sulfhydryl reducing agents. 0.2 mM inhibits 42% Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
Galbeta(1-3)GalNAcalpha-p-nitrophenol pH 7, 37°C, wild-type enzyme Homo sapiens
1.6
-
Galbeta(1-3)GalNAcalpha-p-nitrophenol pH 7, 37°C, mutant enzyme C217S Homo sapiens
1.9
-
UDP-N-acetyl-D-glucosamine pH 7, 37°C, wild-type enzyme Homo sapiens
5.6
-
UDP-N-acetyl-D-glucosamine pH 7, 37°C, mutant enzyme C217S Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for metal ions Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
1 * 58000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Storage Stability

Storage Stability Organism
-80°C, stable for at least 6 months, wild-type enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GalNAcalpha-p-nitrophenol
-
Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 58000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
C2GnT1
-
Homo sapiens
core 2 beta6-N-acetylglucosaminyltransferase
-
Homo sapiens