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Literature summary for 2.4.1.101 extracted from
- Medial Golgi but not late Golgi glycosyltransferases exist as high molecular weight complexes. Role of luminal domain in complex formation and localization (2000), J. Biol. Chem., 275, 11836-11845.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional expression of c-Myc-tagged full length clone and chimera TfR/GnT1myc and exchange mutants of the latter in CHO Lec 1 cells, lacking GnT-1 activity | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D77N | exchange mutation in chimera TfR/GnT1myc, no effect on Golgi localization and inclusion into high molecular weight complexes, no catalytic activity | Homo sapiens |
| additional information | a truncated, soluble enzyme form, consisting of stem region and catalytic domain, is accumulated in the Golgi apparatus prior to secretion | Homo sapiens |
| R83S | exchange mutation in chimera TfR/GnT1myc, no effect on Golgi localization and inclusion into high molecular weight complexes, no catalytic activity | Homo sapiens |
| R85S | exchange mutation in chimera TfR/GnT1myc, no effect on Golgi localization and inclusion into high molecular weight complexes, no catalytic activity | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | medial Golgi enzyme, exists as high molecular weight complex, which do not require the transmembrane domain nor the cytoplasmic tail of the enzyme for complex formation | Homo sapiens | 5794 | - |
| membrane | membrane-bound recombinant chimeras | Homo sapiens | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme exists as high molecular weight complex, which do not require the transmembrane domain nor the cytoplasmic tail of the enzyme for complex formation | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | O-glycosylated | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + ovalbumin | - |
Homo sapiens | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GnT-I | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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