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Literature summary for 2.4.1.101 extracted from

  • Wagner, R.; Liedtke, S.; Kretzschmar, E.; Geyer, H.G.; Geyer, R.; Klenk, H.D.
    Elongation of the N-glycans of fowl plague virus hemagglutinin expressed in Spodoptera frugiperda (Sf9) cells by coexpression of human beta1,2-N-acetylglucosaminyltransferase I (1996), Glycobiology, 6, 165-175.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
functional expression of unmodified enzyme and as tagged fusion protein in Spodoptera frugiperda Sf9 cells via baculovirus infection, coexpression of fowl plague virus hemagglutinin HA as endogenous substrate for in vivo activity assay Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus resident type II transmembrane protein Homo sapiens 5794
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, recombinant enzyme, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 in vivo assay of recombinant enzyme expressed in Sf9 cells with coexpressed fowl plague virus HA as endogenous substrate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 Homo sapiens UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R transfers an N-acetylglucosamine in beta-1,2-linkage to mannosyl-1,3-beta-mannosyl-terminus ?
UDP-N-acetyl-D-glucosamine + fowl plague virus hemagglutinin recombinant coexpression of enzyme and substrate in SF9 insect cells Homo sapiens UDP + fowl plague virus hemagglutinin with terminal N-acetyl-D-glucosaminyl residues
-
 ?
UDP-N-acetyl-D-glucosamine + ovalbumin best substrate, recombinant enzyme expressed in SF9 cells, unmodified and as tagged fusion protein Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
GnT-I
-
 Homo sapiens