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Literature summary for 2.4.1.100 extracted from

  • Verspreet, J.; Cimini, S.; Vergauwen, R.; Dornez, E.; Locato, V.; Le Roy, K.; De Gara, L.; Van den Ende, W.; Delcour, J.A.; Courtin, C.M.
    Fructan metabolism in developing wheat (Triticum aestivum L.) kernels (2013), Plant Cell Physiol., 54, 2047-2057.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Triticum aestivum
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Triticum aestivum Q4AEI9 var. Homeros
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Source Tissue

Source Tissue Comment Organism Textmining
kernel
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Triticum aestivum
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kernel developing from anthesis until maturity. 1-FFT is almost exclusively active during the first 9 days after anthesis and no 1,1-kestotetraose is produced during the last weeks of kernel development Triticum aestivum
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-kestotriose + 6G-kestotriose formation of 1,1-kestotetraose from 1-kestotriose as the single substrate by catalyzing this reaction by transferring fructose from a 1-kestotriose donor to a 6G-kestotriose acceptor Triticum aestivum 1,1-kestotetraose + ?
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Synonyms

Synonyms Comment Organism
1-FFT
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Triticum aestivum
fructan:fructan 1-fructosyltransferase
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Triticum aestivum

General Information

General Information Comment Organism
metabolism comparative study of all major fructan-metabolizing enzymes together with fructan content, fructan degree of polymerization and the presence of fructan oligosaccharides in developing wheat kernels, overview. Fructan synthesis is catalyzed by sucrose:sucrose 1-fructosyltransferase, EC 2.4.1.99, and sucrose:fructan 6-fructosyltransferase, EC 2.4.1.10, and to a lesser extent by fructan:fructan 1-fructosyltransferase, EC 2.4.1.100 Triticum aestivum
physiological function fructan accumulation occurs mainly in the first 2 weeks after anthesis, and a maximal fructan concentration of 2.5 mg fructan per kernel is reached at 16 days after anthesis. Fructan synthesis is catalyzed by 1-SST (sucrose:sucrose 1-fructosyltransferase) and 6-SFT (sucrose:fructan 6-fructosyltransferase), and to a lesser extent by 1-FFT (fructan:fructan 1-fructosyltransferase). Despite the presence of 6G-kestotriose in wheat kernel extracts, the measured 6G-FFT (fructan:fructan 6G-fructosyltransferase) activity levels are low Triticum aestivum