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Literature summary for 2.4.1.10 extracted from
- Fully automated chip-based negative mode nanoelectrospray mass spectrometry of fructooligosaccharides produced by heterologously expressed levansucrase from Pseudomonas syringae pv. tomato DC3000 (2009), Rapid Commun. Mass Spectrom., 23, 1337-1346.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene lsc3, expression in Escherichia coli | Pseudomonas syringae pv. tomato |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pseudomonas syringae pv. tomato | isozyme Lsc3 synthesizes a high molecular weight fructan polymer, levan, from sucrose, but it also shows formation of fructooligosaccharides with potential prebiotic effects from both sucrose and raffinose, degrees of polymerization up to five, overview. Formation of fructooligosaccharides is enhanced by increased sucrose or raffinose concentrations of 600 mM and higher. Analysis of product in underivatized form by fully automated chip-based nanoelectrospray ionization, nanoESI, high-capacity ion trap mass spectrometry, HCT-MS. Structural analysis by tandem mass spectrometry, MS/MS, employing collision-induced dissociation at low energies, overview | ? | - |
? |  |
| additional information | Pseudomonas syringae pv. tomato DC3000 | isozyme Lsc3 synthesizes a high molecular weight fructan polymer, levan, from sucrose, but it also shows formation of fructooligosaccharides with potential prebiotic effects from both sucrose and raffinose, degrees of polymerization up to five, overview. Formation of fructooligosaccharides is enhanced by increased sucrose or raffinose concentrations of 600 mM and higher. Analysis of product in underivatized form by fully automated chip-based nanoelectrospray ionization, nanoESI, high-capacity ion trap mass spectrometry, HCT-MS. Structural analysis by tandem mass spectrometry, MS/MS, employing collision-induced dissociation at low energies, overview | ? | - |
? | |
| sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside | Pseudomonas syringae pv. tomato | - |
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside | - |
? | |
| sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside | Pseudomonas syringae pv. tomato DC3000 | - |
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas syringae pv. tomato | Q88BN6 | isozyme Lsc3, encoded by gene lsc3 | - |
| Pseudomonas syringae pv. tomato DC3000 | Q88BN6 | isozyme Lsc3, encoded by gene lsc3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant isozyme Lsc3 from Escherichia coli by ammonium sulfate fractionation and gel filtration | Pseudomonas syringae pv. tomato |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 230 | - |
purified recombinant enzyme | Pseudomonas syringae pv. tomato |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | isozyme Lsc3 synthesizes a high molecular weight fructan polymer, levan, from sucrose, but it also shows formation of fructooligosaccharides with potential prebiotic effects from both sucrose and raffinose, degrees of polymerization up to five, overview. Formation of fructooligosaccharides is enhanced by increased sucrose or raffinose concentrations of 600 mM and higher. Analysis of product in underivatized form by fully automated chip-based nanoelectrospray ionization, nanoESI, high-capacity ion trap mass spectrometry, HCT-MS. Structural analysis by tandem mass spectrometry, MS/MS, employing collision-induced dissociation at low energies, overview | Pseudomonas syringae pv. tomato | ? | - |
? | |
| additional information | isozyme Lsc3 synthesizes a high molecular weight fructan polymer, levan, from sucrose, but it also shows formation of fructooligosaccharides with potential prebiotic effects from both sucrose and raffinose, degrees of polymerization up to five, overview. Formation of fructooligosaccharides is enhanced by increased sucrose or raffinose concentrations of 600 mM and higher. Analysis of product in underivatized form by fully automated chip-based nanoelectrospray ionization, nanoESI, high-capacity ion trap mass spectrometry, HCT-MS. Structural analysis by tandem mass spectrometry, MS/MS, employing collision-induced dissociation at low energies, overview | Pseudomonas syringae pv. tomato DC3000 | ? | - |
? | |
| sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside | - |
Pseudomonas syringae pv. tomato | D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside | - |
? | |
| sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside | sucrose is used as a starter unit to which fructose residues, cleaved from another sucrose molecule, become attached | Pseudomonas syringae pv. tomato | D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside | - |
? | |
| sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside | - |
Pseudomonas syringae pv. tomato DC3000 | D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside | - |
? | |
| sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside | sucrose is used as a starter unit to which fructose residues, cleaved from another sucrose molecule, become attached | Pseudomonas syringae pv. tomato DC3000 | D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Lsc3 | - |
Pseudomonas syringae pv. tomato |
| sucrose:2,6-beta-D-fructan:6-beta-D-fructosyltransferase | - |
Pseudomonas syringae pv. tomato |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pseudomonas syringae pv. tomato |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Pseudomonas syringae pv. tomato |
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