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Literature summary for 2.4.1.10 extracted from

  • El-Refai, H.; Abdel-Fattah, A.; Mostafa, F.
    Immobilization and properties of Bacillus circulans levansucrase (2009), Acta Pharm. Sci., 51, 149-156.
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
additional information enzyme immobilization on wool, leading to higher optimal reaction temperature, low activation energy, higher Km and lower Vmax, compared to the free enzyme, with thermal stability and resistance to chemical denaturation, overview. The immobilized enzyme shows 78.12% of the free enzyme's specific activity Niallia circulans

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 20 ng/ml medium, 42% inhibition of the free enzyme, 80% inhibition of the immobilized enzyme Niallia circulans
Fe3+ 20 ng/ml medium, 30% inhibition of the free enzyme, 22% inhibition of the immobilized enzyme Niallia circulans
Hg2+ 20 ng/ml medium, 67.5% inhibition of the free enzyme, 54.3% inhibition of the immobilized enzyme Niallia circulans
Mn2+ 20 ng/ml medium, 80% inhibition of the free enzyme, 75% inhibition of the immobilized enzyme Niallia circulans
Zn2+ 20 ng/ml medium, 75% inhibition of the free enzyme, 70% inhibition of the immobilized enzyme Niallia circulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of free and immobilized enzymes, overview Niallia circulans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Niallia circulans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme activities of free and immobilized enzymes are not affected by Ca2+ Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme from cell culture supernatant by ethanol precipitation with 50% v/v ethanol Niallia circulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.82
-
partially purified free enzyme Niallia circulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40 free and immobilized enzymes Niallia circulans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50
-
Niallia circulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
free and immobilized enzymes, 60 min, pH 5.2, completely stable Niallia circulans
50
-
60 min, pH 5.2, the free enzyme loses 50% activity, the immobilized enzyme loses 40% activity Niallia circulans
60
-
60 min, pH 5.2, the free enzyme loses over 90% activity, the immobilized enzyme loses 80% activity Niallia circulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
free enzyme Niallia circulans
5.6
-
immobilized enzyme Niallia circulans

pH Range

pH Minimum pH Maximum Comment Organism
4.4 6.8 activity range of free and immobilized enzymes, with higher activity of the immobilized enzyme, profiles, overview Niallia circulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.4 6.8 25°C, immobilized and free enzyme, 60 min, highest stability at pH 5.0-6.0, profiles, overview Niallia circulans