Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme immobilization on wool, leading to higher optimal reaction temperature, low activation energy, higher Km and lower Vmax, compared to the free enzyme, with thermal stability and resistance to chemical denaturation, overview. The immobilized enzyme shows 78.12% of the free enzyme's specific activity | Niallia circulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 20 ng/ml medium, 42% inhibition of the free enzyme, 80% inhibition of the immobilized enzyme | Niallia circulans | |
Fe3+ | 20 ng/ml medium, 30% inhibition of the free enzyme, 22% inhibition of the immobilized enzyme | Niallia circulans | |
Hg2+ | 20 ng/ml medium, 67.5% inhibition of the free enzyme, 54.3% inhibition of the immobilized enzyme | Niallia circulans | |
Mn2+ | 20 ng/ml medium, 80% inhibition of the free enzyme, 75% inhibition of the immobilized enzyme | Niallia circulans | |
Zn2+ | 20 ng/ml medium, 75% inhibition of the free enzyme, 70% inhibition of the immobilized enzyme | Niallia circulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of free and immobilized enzymes, overview | Niallia circulans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Niallia circulans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme activities of free and immobilized enzymes are not affected by Ca2+ | Niallia circulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme from cell culture supernatant by ethanol precipitation with 50% v/v ethanol | Niallia circulans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.82 | - |
partially purified free enzyme | Niallia circulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | free and immobilized enzymes | Niallia circulans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | - |
Niallia circulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
free and immobilized enzymes, 60 min, pH 5.2, completely stable | Niallia circulans |
50 | - |
60 min, pH 5.2, the free enzyme loses 50% activity, the immobilized enzyme loses 40% activity | Niallia circulans |
60 | - |
60 min, pH 5.2, the free enzyme loses over 90% activity, the immobilized enzyme loses 80% activity | Niallia circulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
free enzyme | Niallia circulans |
5.6 | - |
immobilized enzyme | Niallia circulans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.4 | 6.8 | activity range of free and immobilized enzymes, with higher activity of the immobilized enzyme, profiles, overview | Niallia circulans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.4 | 6.8 | 25°C, immobilized and free enzyme, 60 min, highest stability at pH 5.0-6.0, profiles, overview | Niallia circulans |