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Literature summary for 2.4.1.10 extracted from

  • Ozimek, L.K.; Euverink, G.J.; van der Maarel, M.J.; Dijkhuizen, L.
    Mutational analysis of the role of calcium ions in the Lactobacillus reuteri strain 121 fructosyltransferase (levansucrase and inulosucrase) enzymes (2005), FEBS Lett., 579, 1124-1128.
    View publication on PubMed
Search for more literature for 2.4.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Limosilactobacillus reuteri

Protein Variants

Protein Variants Comment Organism
D500A at 45°C the mutant enzyme is inactive in absence of Ca2+, 45% of wild-type activity remains in presence of 1 mM Ca2+ Limosilactobacillus reuteri
D500A at 45°C the mutant enzyme is inactive in the absence of Ca2+ ions, with 15% of wild-type activity remaining in the presence of 1 mM Ca2+. In the presence of 1 mM Ca2+ mutant enzyme displays highest activity at 40°C. In the absence of Ca2+ ions, the optimal temperature is 30°C Limosilactobacillus reuteri
D500N at 45°C the mutant enzyme is inactive in absence of Ca2+, 45% of wild-type activity remains in presence of 1 mM Ca2+ Limosilactobacillus reuteri
D500N at 45°C the mutant enzyme is inactive in the absence of Ca2+ ions, with 45% of wild-type activity remaining in the presence of 1 mM Ca2+. In the presence of 1 mM Ca2+ mutant enzyme displays highest activity at 40°C. In the absence of Ca2+ ions, the optimal temperature is 30°C Limosilactobacillus reuteri

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+ play an important structural role. At 45°C the mutant enzymes D500A and D500N are inactive in the absence of Ca2+ ions, with, respectively, 15% and 45% of wild-type activity remaining in the presence of 1 mM Ca2+. In the presence of 1 mM Ca2+ mutant enzymes display highest activity at 40°C. In the absence of Ca2+ ions, the optimal temperature is 30°C Limosilactobacillus reuteri
Ca2+ stimulates activity, Asp500 residues play an important role in Ca2+ binding Limosilactobacillus reuteri

Organism

Organism UniProt Comment Textmining
Limosilactobacillus reuteri
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Limosilactobacillus reuteri Q8GGV4
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Limosilactobacillus reuteri 121
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Limosilactobacillus reuteri 121 Q8GGV4
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Purification (Commentary)

Purification (Comment) Organism
-
 Limosilactobacillus reuteri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
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 in the absence of Ca2+ ions, the optimal temperature of mutant enzyme D500N and D500A is 30°C Limosilactobacillus reuteri
30
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 maximal activity of mutant enzymes D500N and D500A in absence of Ca2+ Limosilactobacillus reuteri
40
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 in the presence of 1 mM Ca2+ mutant enzymes D500N and D500A display highest activity at 40°C Limosilactobacillus reuteri
40
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 maximal activity of mutant enzymes D500N and D500A in presence of 1 mM Ca2+ Limosilactobacillus reuteri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
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 Ca2+ protects against thermal inactivation Limosilactobacillus reuteri