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Literature summary for 2.4.1.10 extracted from

  • Tanaka, T.; Oi, S.; Iizuka, M.; Yamamoto, T.
    Levansucrase of Bacillus subtilis (1978), Agric. Biol. Chem., 42, 323-326.
No PubMed abstract available

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
var. saccharolyticus
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n
-
Bacillus subtilis glucose + (2,6-beta-D-fructosyl)n+1
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
at 0°C the enzyme is more productive over a period of 10 h, but the enzyme activity is higher at 37°C Bacillus subtilis
-
-
at 0°C the enzyme is more productive over a peroid of 10 h, but the enzyme activity is higher at 37°C Bacillus subtilis
30
-
assay at Bacillus subtilis
37
-
at 0°C the enzyme is more productive over a peroid of 10 h, but the enzyme activity is higher at 37°C Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
15 min, pH 7.0, loss of activity Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
hydrolysis of sucrose to glucose and fructose Bacillus subtilis
7
-
assay at Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 7.5 4°C, stable Bacillus subtilis