Cloned (Comment) | Organism |
---|---|
heterologous gene expression of enzyme harboring Strep-Tag II fused to the C-terminal Tyr-465 of the enzyme, in Thermococcus kodakaraensis | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
phosphate | 70°C, pH 7.0 | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE | Saccharolobus solfataricus |
69000 | - |
gel filtration, recombinant protein | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
similar specific activity for substrates maltodextrin, maltopentaose and starch, 70°C, pH 7.0 | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(maltodextrin)n + phosphate | - |
Saccharolobus solfataricus | (maltodextrin)n-1 + alpha-D-glucose 1-phosphate | - |
? | |
maltopentaose + phosphate | - |
Saccharolobus solfataricus | maltotetraose + alpha-D-glucose 1-phosphate | - |
? | |
maltotetraose + phosphate | 50% of the activity with maltopentaose | Saccharolobus solfataricus | maltotriose + alpha-D-glucose 1-phosphate | - |
? | |
additional information | no substrate in direction of glucose 1-phosphate synthesis: pullulan, maltotriose | Saccharolobus solfataricus | ? | - |
? | |
starch + alpha-D-glucose 1-phosphate | - |
Saccharolobus solfataricus | starch + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
phosphate | 70°C, pH 7.0 | Saccharolobus solfataricus |