Cloned (Comment) | Organism |
---|---|
gene glgP, DNA sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, forward and reverse reaction, recombinant enzyme | Aquifex aeolicus | |
0.17 | - |
maltopentaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
0.18 | - |
maltohexaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
0.19 | - |
maltoheptaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
0.28 | - |
maltopentaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
0.3 | - |
maltotetraose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
0.3 | - |
maltohexaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
0.38 | - |
maltoheptaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
0.55 | - |
maltotetraose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
1.8 | - |
maltotriose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, recombinant His-tagged enzyme, SDS-PAGE, x * 81981, sequence calculation | Aquifex aeolicus |
81981 | - |
x * 80000, recombinant His-tagged enzyme, SDS-PAGE, x * 81981, sequence calculation | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1,4-alpha-D-glucosyl)n + phosphate | Aquifex aeolicus | polysaccharide substrate is glycogen, mobilization of glucose 1-phosphate as readily useable energy source | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O66932 | gene glgP | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli 6.3fold to homogeneity by heat treatment at 70°C and nickel affinity chromatography | Aquifex aeolicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | sequential random bi bi mechanism | Aquifex aeolicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.8 | - |
purified recombinant enzyme | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1,4-alpha-D-glucosyl)n + phosphate | polysaccharide substrate is glycogen, mobilization of glucose 1-phosphate as readily useable energy source | Aquifex aeolicus | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
(1,4-alpha-D-glucosyl)n + phosphate | polysaccharide substrates are glycogen and starch, phosphorolysis is the preferred reaction | Aquifex aeolicus | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
maltoheptaose + alpha-D-glucose 1-phosphate | - |
Aquifex aeolicus | maltooctaose + phosphate | - |
r | |
maltohexaose + alpha-D-glucose 1-phosphate | - |
Aquifex aeolicus | maltoheptaose + phosphate | - |
r | |
maltopentaose + alpha-D-glucose 1-phosphate | - |
Aquifex aeolicus | maltohexaose + phosphate | - |
r | |
maltotetraose + alpha-D-glucose 1-phosphate | - |
Aquifex aeolicus | maltopentaose + phosphate | - |
r | |
maltotriose + alpha-D-glucose 1-phosphate | maltotriose is the smallest substrate accepted for the synthetic reaction direction, maltotetraose is the smallest substrate accepted for the phosphorolytic reaction direction | Aquifex aeolicus | maltotetraose + phosphate | - |
r | |
additional information | maltotriose is the smallest substrate accepted for the synthetic reaction direction, maltotetraose is the smallest substrate accepted for the phosphorolytic reaction direction | Aquifex aeolicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, recombinant His-tagged enzyme, SDS-PAGE, x * 81981, sequence calculation | Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
glycogen phosphorylase | - |
Aquifex aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
recombinant enzyme | Aquifex aeolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
84% remaining activity after 30 min, recombinant enzyme | Aquifex aeolicus |
100 | - |
66% remaining activity after 30 min, recombinant enzyme | Aquifex aeolicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
maltotetraose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
2.2 | - |
maltopentaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
2.3 | - |
maltoheptaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
2.7 | - |
maltohexaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
4.1 | - |
maltohexaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
4.4 | - |
maltoheptaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
5.4 | - |
maltopentaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
6 | - |
maltotetraose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
6.8 | - |
maltotriose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
recombinant enzyme | Aquifex aeolicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | recombinant enzyme, stable, 61% remaining activity at 70°C and pH 10.4 | Aquifex aeolicus |