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Literature summary for 2.4.1.1 extracted from
- Purification and characterization of starch phosphorylase from Cuscuta reflexa filaments (1995), Phytochemistry, 39, 1001-1005.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by aromatic compounds | Cuscuta reflexa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.19 | - |
glucose 1-phosphate | at 3.1 mM starch | Cuscuta reflexa |  |
| 1.25 | - |
glucose 1-phosphate | at 6.2 mM starch | Cuscuta reflexa | |
| 1.38 | - |
glucose 1-phosphate | at 18.6 mM starch | Cuscuta reflexa | |
| 4.4 | - |
starch | at 0.5 mM glucose 1-phosphate | Cuscuta reflexa | |
| 5.6 | - |
starch | at 1.0 mM glucose 1-phosphate | Cuscuta reflexa | |
| 15.5 | - |
starch | at 5 mM glucose 1-phosphate | Cuscuta reflexa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
4 * 100000, SDS-PAGE | Cuscuta reflexa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cuscuta reflexa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-cellulose, Sephadex G-200 | Cuscuta reflexa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| filament | - |
Cuscuta reflexa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.96 | - |
- |
Cuscuta reflexa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + glucose 1-phosphate | - |
Cuscuta reflexa | starch + phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 100000, SDS-PAGE | Cuscuta reflexa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
starch synthesis | Cuscuta reflexa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | - |
starch synthesis, half-maximal activity at pH 5.2 and pH 7.8 | Cuscuta reflexa |
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