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Literature summary for 2.4.1.1 extracted from
- Isolation and partial characterization of two forms of rat heart glycogen phosphorylase (1984), Arch. Biochem. Biophys., 228, 143-154.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 5'-AMP | activation | Rattus norvegicus |  |
| 5'-AMP | half-maximal activation of phosphorylase Ib at 0.022 mM, 0.007 mM for phosphorylase IIb | Rattus norvegicus | |
| phosphate | activation | Rattus norvegicus | |
| phosphate | converts phosphorylase b into phosphorylase a | Rattus norvegicus | |
| Phosphorylase kinase | activation | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Caffeine | 5 mM, 95% inhibition of heart phosphorylase Ib in the presence of 1 mM AMP, 30% inhibition of heart phosphorylase IIb | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [(1->4)-alpha-D-glucosyl]n + phosphate | Rattus norvegicus | glycogen metabolism | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Wistar | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | incorporation of approx. 1 mol phosphate/mol of subunit | Rattus norvegicus |
| side-chain modification | activation by phosphorylation | Rattus norvegicus |
| side-chain modification | phosphorylation converts inactive phosphorylase b into active phosphorylase a | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| heart isozymes I and II, ammonium sulfate, DEAE-Sephacel, AMP-Sepharose, muscle enzyme, ammonium sulfate, DEAE-Sephacel | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Rattus norvegicus | - |
| muscle | skeletal muscle | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 46 | - |
heart isoenzymes I and II | Rattus norvegicus |
| 51.6 | - |
skeletal muscle | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol, at least 4 months | Rattus norvegicus |
| glycerol stabilizes purified enzyme during storage | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycogen + glucose 1-phosphate | - |
Rattus norvegicus | glycogen + phosphate | - |
r | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | - |
Rattus norvegicus | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| [(1->4)-alpha-D-glucosyl]n + phosphate | glycogen metabolism | Rattus norvegicus | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 6.2, skeletal muscle, heart isozyme Ib | Rattus norvegicus |
| additional information | - |
pI: 5.2, heart isozyme IIb | Rattus norvegicus |
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