Cloned (Comment) | Organism |
---|---|
His-tag version expressed in Escherichia coli | Escherichia coli |
His-tagged version, gene synthesized commercially, expressed in Escherichia coli | Bacillus anthracis |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Bacillus anthracis |
Protein Variants | Comment | Organism |
---|---|---|
C438S | residue in the acetyl-CoA binding pocket | Escherichia coli |
C617S | residue in the acetyl-CoA binding pocket | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4,5-trioxopyrrolidine-3-carboxamide | competitive | Escherichia coli | |
oxalate | competitive | Escherichia coli | |
parabanic acid | competitive | Escherichia coli | |
pyruvate | - |
Bacillus anthracis | |
pyruvate | competitive | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
acetyl-CoA | wild-type protein | Escherichia coli | |
0.021 | - |
glyoxylate | wild-type protein | Escherichia coli | |
0.022 | - |
acetyl-CoA | wild-type protein | Escherichia coli | |
0.023 | - |
acetyl-CoA | - |
Bacillus anthracis | |
0.046 | - |
acetyl-CoA | C617 mutant protein | Escherichia coli | |
0.05 | - |
glyoxylate | C617 mutant protein | Escherichia coli | |
0.055 | - |
glyoxylate | wild-type protein | Escherichia coli | |
0.056 | - |
acetyl-CoA | C438S mutant protein | Escherichia coli | |
0.058 | - |
glyoxylate | C438S mutant protein | Escherichia coli | |
0.085 | - |
glyoxylate | - |
Bacillus anthracis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli | |
Mg2+ | - |
Bacillus anthracis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | A0A0F7RL08 | - |
- |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Bacillus anthracis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
C438S mutant protein has 74% of wild-type acitvity | Escherichia coli |
additional information | - |
C617S mutant protein has 88% of wild-type acitvity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + glyoxylate + H2O | - |
Escherichia coli | CoA + (S)-malate | - |
? | |
acetyl-CoA + glyoxylate + H2O | - |
Bacillus anthracis | CoA + (S)-malate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
malate synthase A | - |
Escherichia coli |
malate synthase A | - |
Bacillus anthracis |
malate synthase G | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
oxalate | wild-type protein | Escherichia coli | |
0.037 | - |
oxalate | wild-type protein | Escherichia coli | |
0.04 | - |
2,4,5-trioxopyrrolidine-3-carboxamide | C438S mutant protein | Escherichia coli | |
0.113 | - |
2,4,5-trioxopyrrolidine-3-carboxamide | C617 mutant protein | Escherichia coli | |
0.37 | - |
parabanic acid | C438S mutant protein | Escherichia coli | |
0.55 | - |
parabanic acid | C617 mutant protein | Escherichia coli | |
0.6 | - |
pyruvate | C438S mutant protein | Escherichia coli | |
0.65 | - |
pyruvate | wild-type protein | Escherichia coli | |
0.75 | - |
pyruvate | - |
Bacillus anthracis | |
1 | - |
pyruvate | C617 mutant protein | Escherichia coli | |
1 | - |
pyruvate | wild-type protein | Escherichia coli |