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Literature summary for 2.3.3.9 extracted from
- Purification and characterization of a cold-adapted isocitrate lyase and a malate synthase from Colwellia maris, a psychrophilic bacterium (2001), Biosci. Biotechnol. Biochem., 65, 1095-1103.
General Stability
| General Stability | Organism |
|---|---|
| DTT and glycerol stabilize | Colwellia maris |
| DTT stabilizes | Colwellia maris |
| salt does not stabilize | Colwellia maris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | - |
Colwellia maris |  |
| NaCl | - |
Colwellia maris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0198 | - |
glyoxylate | - |
Colwellia maris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | about 35% of the activation with Mg2+ | Colwellia maris | |
| Mg2+ | required | Colwellia maris | |
| Mn2+ | 19% of the activation with Mg2+ | Colwellia maris | |
| Ni2+ | about 98% of the activation with Mg2+ | Colwellia maris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
gel filtration | Colwellia maris |
| 763000 | - |
2 * 763000, SDS-PAGE | Colwellia maris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + acetyl-CoA + H2O | Colwellia maris | enzyme specifically involved in glyoxalate cycle metabolism | (S)-malate + CoA | - |
? | |
| glyoxylate + acetyl-CoA + H2O | Colwellia maris | enzyme essential for acetate use in the bacterial cells | (S)-malate + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Colwellia maris | - |
formerly Vibrio sp. strain ABE-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Colwellia maris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:acetate-grown cell | - |
Colwellia maris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 69.6 | - |
- |
Colwellia maris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for more than one month | Colwellia maris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + acetyl-CoA + H2O | - |
Colwellia maris | (S)-malate + CoA | - |
? | |
| glyoxylate + acetyl-CoA + H2O | enzyme specifically involved in glyoxalate cycle metabolism | Colwellia maris | (S)-malate + CoA | - |
? | |
| glyoxylate + acetyl-CoA + H2O | enzyme essential for acetate use in the bacterial cells | Colwellia maris | (S)-malate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 763000, SDS-PAGE | Colwellia maris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Colwellia maris |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Km for glyoxylate increases with decreasing temperature | Colwellia maris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 60.7 | - |
acetyl-CoA | at 20°C | Colwellia maris | |
| 161 | - |
acetyl-CoA | at 45°C | Colwellia maris | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Colwellia maris |
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