Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | activation | Gossypium hirsutum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetaldehyde | weak | Gossypium hirsutum | |
ADP | - |
Gossypium hirsutum | |
ATP | - |
Gossypium hirsutum | |
Butanedione | slight inhibition at high concentrations | Gossypium hirsutum | |
chloroacetyl-CoA | - |
Gossypium hirsutum | |
deamino-acetyl-CoA | - |
Gossypium hirsutum | |
deoxycholate | - |
Gossypium hirsutum | |
dephosphoacetyl-CoA | - |
Gossypium hirsutum | |
diethyl dicarbonate | inhibition prevented by preincubation with acetyl-CoA | Gossypium hirsutum | |
glycolate | - |
Gossypium hirsutum | |
methylglyoxal | - |
Gossypium hirsutum | |
oxalate | - |
Gossypium hirsutum | |
oxamate | - |
Gossypium hirsutum | |
pyridoxal-5'-phosphate | preincubation with glyoxylate but not acetyl-CoA prevents inhibition | Gossypium hirsutum | |
pyruvate | weak | Gossypium hirsutum | |
S-acetonyl-CoA | - |
Gossypium hirsutum | |
S-ethyl-CoA | - |
Gossypium hirsutum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
acetyl-CoA | - |
Gossypium hirsutum | |
0.052 | - |
glyoxylate | - |
Gossypium hirsutum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | about 30% of the activation with Mg2+ | Gossypium hirsutum | |
Co2+ | about 30% of the activation with Mg2+ | Gossypium hirsutum | |
Mg2+ | Km: 0.59 mM | Gossypium hirsutum | |
Mg2+ | absolute requirement for divalent metal ion, best fulfilled by Mg2+ | Gossypium hirsutum | |
Mn2+ | about 30% of the activation with Mg2+ | Gossypium hirsutum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
730000 | - |
gel filtration | Gossypium hirsutum |
750000 | - |
sucrose density gradient centrifugation | Gossypium hirsutum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + acetyl-CoA + H2O | Gossypium hirsutum | enzyme specifically involved in glyoxalate cycle metabolism | (S)-malate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gossypium hirsutum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gossypium hirsutum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA | sequential bireactant mechanism | Gossypium hirsutum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | of dark-germinated seeds | Gossypium hirsutum | - |
seed | - |
Gossypium hirsutum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Gossypium hirsutum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + acetyl-CoA + H2O | - |
Gossypium hirsutum | (S)-malate + CoA | - |
ir | |
glyoxylate + acetyl-CoA + H2O | enzyme specifically involved in glyoxalate cycle metabolism | Gossypium hirsutum | (S)-malate + CoA | - |
? | |
glyoxylate + butyryl-CoA + H2O | - |
Gossypium hirsutum | 2-oxohexanedioic acid + CoA | - |
ir | |
glyoxylate + propionyl-CoA + H2O | - |
Gossypium hirsutum | 2-oxopentanedioic acid + CoA | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Gossypium hirsutum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
2 min, 84% loss of activity | Gossypium hirsutum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
Tris/HCl buffer | Gossypium hirsutum |
8.2 | - |
phosphate-citrate-borate buffer or MOPS-KOH buffer | Gossypium hirsutum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 9.7 | pH 6.8 and 9.7: about 50% of maximal activity, phosphate-citrate-borate buffer | Gossypium hirsutum |