Literature summary for 2.3.3.8 extracted from

Fan, F.; Williams, H.J.; Boyer, J.G.; Graham, T.L.; Zhao, H.; Lehr, R.; Qi, H.; Schwartz, B.; Raushel, F.M.; Meek, T.D.
On the catalytic mechanism of human ATP citrate lyase (2012), Biochemistry, 51, 5198-5211.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Sf9 cells using a a baculovirus expression system	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
H760A	the turnover number of the mutant is similar to that of wild type in the absence of citrate and CoA, yet significantly less than that of wild type enzyme in the presence of both citrate and CoA	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
diethyldicarbonate	the addition of 0.5 mM ATP in the preincubation reaction mixture provided complete protection of enzyme activity from inactivation by diethyldicarbonate	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
110000	-	4 * 110000, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Superose 12 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + citrate + CoA	-	Homo sapiens	ADP + phosphate + acetyl-CoA + oxaloacetate	-	r

Subunits

Subunits	Comment	Organism
homotetramer	4 * 110000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
ACL	-	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
19	-	ATP	wild type enzyme, average value, in 50 mM Tris, 10 mM MgCl2, and 2 mM dithiothreitol (pH 8.0), at 22°C	Homo sapiens
230	-	citrate	wild type enzyme, average value, in 50 mM Tris, 10 mM MgCl2, and 2 mM dithiothreitol (pH 8.0), at 22°C	Homo sapiens
240	-	CoA	wild type enzyme, average value, in 50 mM Tris, 10 mM MgCl2, and 2 mM dithiothreitol (pH 8.0), at 22°C	Homo sapiens

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Release 2023.1 (January 2023)