Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.3.5 extracted from

  • Ewering, C.; Heuser, F.; Benoelken, J.K.; Braemer, C.O.; Steinbuechel, A.
    Metabolic engineering of strains of Ralstonia eutropha and Pseudomonas putida for biotechnological production of 2-methylcitric acid (2006), Metab. Eng., 8, 587-602.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the chromosomal gene encoding the 2-methyl-cis-aconitate hydratase is disrupted by direct insertion of a copy of an additional 2-methylcitrate synthase gene (prpc). 2-Methylcitrate synthase is expressed under the control of the constitutive kanamycin-resistance gene resulting in up to 20fold higher specific 2-methylcitrate synthase activities in comparison to the wild-type. The disruption of the acnM gene by insertion of prpC leads to a propionate- and levulinate-negative phenotype of the engineered strains and accumulation of 2-methylcitrate in the medium Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-