Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | 5 mM AMP increases the enzyme activity to about 23% | Streptomyces diastaticus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Streptomyces diastaticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 14% inhibition at 5 mM | Streptomyces diastaticus | |
Ag+ | almost complete inhibition at 2 mM | Streptomyces diastaticus | |
ATP | 32% inhibition at 5 mM | Streptomyces diastaticus | |
Co2+ | 22% inhibition at 2 mM | Streptomyces diastaticus | |
Cu2+ | 10% inhibition at 2 mM | Streptomyces diastaticus | |
Hg2+ | 95% inhibition at 2 mM | Streptomyces diastaticus | |
Mn2+ | 40% inhibition at 2 mM | Streptomyces diastaticus | |
additional information | not influenced by NADH | Streptomyces diastaticus | |
Ni2+ | 21% inhibition at 2 mM | Streptomyces diastaticus | |
Pb2+ | almost complete inhibition at 2 mM | Streptomyces diastaticus | |
Zn2+ | 55% inhibition at 2 mM | Streptomyces diastaticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0112 | - |
oxaloacetate | at pH 8.0 and 35°C | Streptomyces diastaticus | |
0.0584 | - |
acetyl-CoA | at pH 8.0 and 35°C | Streptomyces diastaticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 116.6% activity at 2 mM | Streptomyces diastaticus | |
additional information | the enzyme does not require monovalent or divalent metal ions for catalysis | Streptomyces diastaticus | |
Na+ | 112.6% activity at 2 mM | Streptomyces diastaticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48000, SDS-PAGE | Streptomyces diastaticus |
49868 | - |
2 * 49868, MALDI-TOF mass spectrometry | Streptomyces diastaticus |
99990 | - |
gel filtration | Streptomyces diastaticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | Streptomyces diastaticus | - |
citrate + CoA | - |
? | |
acetyl-CoA + H2O + oxaloacetate | Streptomyces diastaticus SM33 | - |
citrate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces diastaticus | G8XQY5 | - |
- |
Streptomyces diastaticus SM33 | G8XQY5 | - |
- |
Purification (Comment) | Organism |
---|---|
Talon metal (Co2+) affinity resin column chromatography | Streptomyces diastaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | - |
Streptomyces diastaticus | citrate + CoA | - |
? | |
acetyl-CoA + H2O + oxaloacetate | - |
Streptomyces diastaticus SM33 | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 48000, SDS-PAGE | Streptomyces diastaticus |
homodimer | 2 * 49868, MALDI-TOF mass spectrometry | Streptomyces diastaticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Streptomyces diastaticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 55 | the enzyme maintains almost all of its initial activity after incubation for 20 min at 40°C or below. The half-life of the enzyme is 10 min at 50°C and is increased to 210 min in the presence of oxaloacetate. Enzyme activity is undetectable after incubation for 10 min at 55°C | Streptomyces diastaticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
230.7 | - |
oxaloacetate | at pH 8.0 and 35°C | Streptomyces diastaticus | |
262.8 | - |
acetyl-CoA | at pH 8.0 and 35°C | Streptomyces diastaticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Streptomyces diastaticus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4500 | - |
acetyl-CoA | at pH 8.0 and 35°C | Streptomyces diastaticus | |
20700 | - |
oxaloacetate | at pH 8.0 and 35°C | Streptomyces diastaticus |