Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
D113A | mutant with reduce thermostability | Pyrococcus furiosus |
D113S | mutant with reduce thermostability | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43290 | - |
2 * 43290, calculated from amino acid sequence | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q53554 | - |
- |
Purification (Comment) | Organism |
---|---|
Matrex Red Gel A affinity column chromatography | Pyrococcus furiosus |
Renatured (Comment) | Organism |
---|---|
complete recovery of activity is observed after incubation for 1 h at 50°C by a 20fold dilution in 50 mM phosphate buffer of the enzyme fully unfolded in 6 M guanidine-HCl | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | - |
Pyrococcus furiosus | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 43290, calculated from amino acid sequence | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
citrate synthase | - |
Pyrococcus furiosus |