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Literature summary for 2.3.3.16 extracted from

  • Moore, V.; Kanu, A.; Byron, O.; Campbell, G.; Danson, M.J.; Hough, D.W.; Crennell, S.J.
    Contribution of inter-subunit interactions to the thermostability of Pyrococcus furiosus citrate synthase (2011), Extremophiles, 15, 327-336.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
D113A mutant with reduce thermostability Pyrococcus furiosus
D113S mutant with reduce thermostability Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43290
-
2 * 43290, calculated from amino acid sequence Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q53554
-
-

Purification (Commentary)

Purification (Comment) Organism
Matrex Red Gel A affinity column chromatography Pyrococcus furiosus

Renatured (Commentary)

Renatured (Comment) Organism
complete recovery of activity is observed after incubation for 1 h at 50°C by a 20fold dilution in 50 mM phosphate buffer of the enzyme fully unfolded in 6 M guanidine-HCl Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate
-
Pyrococcus furiosus citrate + CoA
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 43290, calculated from amino acid sequence Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
citrate synthase
-
Pyrococcus furiosus