Literature summary for 2.3.3.16 extracted from

Barone, P.; Rosellini, D.; Lafayette, P.; Bouton, J.; Veronesi, F.; Parrott, W.
Bacterial citrate synthase expression and soil aluminum tolerance in transgenic alfalfa (2008), Plant Cell Rep., 27, 893-901.

Cloned(Commentary)

Cloned (Comment)	Organism
alfalfa is engineered by introducing the citrate synthase gene controlled by the Arabidopsis Act2 constitutive promoter or the tobacco RB7 root-specific promoter. Fifteen transgenic plants are assayed for exclusion of aluminum from the root tip, for internal citrate content, for growth in in vitro assays, or for shoot and root growth in either hydroponics or in soil assays. Based on the soil assays, two transgenic events are identified that are more aluminum-tolerant than the non-transgenic control, confirming that citrate synthase overexpression can be a useful tool to help achieve aluminum tolerance	Pseudomonas aeruginosa
overexpression of the CS gene under control of the Arabidopsis thaliana Act2 constitutive promoter or the Nicotiana tabacum RB7 root-specific promoter in alfalfa, Medicago sativa, using the Agrobacterium tumefaciens, strain LBA4404, transformation method	Pseudomonas aeruginosa

Cloned (Comment)

Organism

alfalfa is engineered by introducing the citrate synthase gene controlled by the Arabidopsis Act2 constitutive promoter or the tobacco RB7 root-specific promoter. Fifteen transgenic plants are assayed for exclusion of aluminum from the root tip, for internal citrate content, for growth in in vitro assays, or for shoot and root growth in either hydroponics or in soil assays. Based on the soil assays, two transgenic events are identified that are more aluminum-tolerant than the non-transgenic control, confirming that citrate synthase overexpression can be a useful tool to help achieve aluminum tolerance

Pseudomonas aeruginosa

overexpression of the CS gene under control of the Arabidopsis thaliana Act2 constitutive promoter or the Nicotiana tabacum RB7 root-specific promoter in alfalfa, Medicago sativa, using the Agrobacterium tumefaciens, strain LBA4404, transformation method

Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
additional information	overexpression of Pseudomonas aeruginosa citrate synthase in transgenic alfalfa, Medicago sativa, plants leads to increased soil aluminum tolerance and increased exclusion of Al from the root tip, overview	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-
Pseudomonas aeruginosa	-	gene CS	-

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Release 2023.1 (January 2023)