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Literature summary for 2.3.3.16 extracted from

  • Bond, D.R.; Mester, T.; Nesbo, C.L.; Izquierdo-Lopez, A.V.; Collart, F.L.; Lovley, D.R.
    Characterization of citrate synthase from Geobacter sulfurreducens and evidence for a family of citrate synthases similar to those of eukaryotes throughout the Geobacteraceae (2005), Appl. Environ. Microbiol., 71, 3858-3865.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacter sulfurreducens

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Geobacter sulfurreducens
additional information no inhibition by NADH or 2-oxaloacetate Geobacter sulfurreducens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0043
-
oxaloacetate 30°C, pH 8, native enzyme Geobacter sulfurreducens
0.0081
-
oxaloacetate 30°C, pH 8, recombinant enzyme Geobacter sulfurreducens
0.0123
-
acetyl-CoA 30°C, pH 8, recombinant enzyme Geobacter sulfurreducens
0.0141
-
acetyl-CoA 30°C, pH 8, native enzyme Geobacter sulfurreducens

Organism

Organism UniProt Comment Textmining
Geobacter sulfurreducens Q6JGI2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacter sulfurreducens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
Geobacter sulfurreducens citrate + CoA
-
?
additional information the enzyme does not have any methylcitrate synthase activity Geobacter sulfurreducens ?
-
?

Subunits

Subunits Comment Organism
dimer
-
Geobacter sulfurreducens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.3
-
acetyl-CoA 30°C, pH 8, native enzyme Geobacter sulfurreducens
8.3
-
oxaloacetate 30°C, pH 8, native enzyme Geobacter sulfurreducens
8.8
-
acetyl-CoA 30°C, pH 8, recombinant enzyme Geobacter sulfurreducens
8.8
-
oxaloacetate 30°C, pH 8, recombinant enzyme Geobacter sulfurreducens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Geobacter sulfurreducens

pH Range

pH Minimum pH Maximum Comment Organism
6 10 about 30% of maximal activity at pH 6 and at pH 10 Geobacter sulfurreducens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.9
-
ATP 30°C, pH 8, versus acetyl-CoA Geobacter sulfurreducens

pI Value

Organism Comment pI Value Maximum pI Value
Geobacter sulfurreducens calculation from nucleotide sequence
-
6.46