Literature summary for 2.3.3.16 extracted from

Kurz, L.C.; Drysdale, G.; Riley, M.; Tomar, M.A.; Chen, J.; Russell, R.J.M.; Danson, M.J.
Kinetics and mechanism of the citrate synthase from the thermophilic archaeon Thermoplasma acidophilum (2000), Biochemistry, 39, 2283-2296.

Search for more literature for 2.3.3.16

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	free enzyme forms various binary and ternary complexes with substrates	Thermoplasma acidophilum

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	-	crystalline	-
Thermoplasma acidophilum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from E. coli	Thermoplasma acidophilum

Reaction

Reaction	Comment	Organism	Reaction ID
acetyl-CoA + H2O + oxaloacetate = citrate + CoA	active site	Sus scrofa	a
acetyl-CoA + H2O + oxaloacetate = citrate + CoA	active site	Thermoplasma acidophilum	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
heart	-	Sus scrofa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + oxaloacetate + H2O	condensation step nearly irreversible, reversibility is increased at 70°C	Thermoplasma acidophilum	citrate + CoA	-	r
acetyl-CoA + oxaloacetate + H2O	condensation step is reversible	Sus scrofa	citrate + CoA	-	r

Synonyms

Synonyms	Comment	Organism
CS	-	Sus scrofa
CS	-	Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Sus scrofa
70	-	-	Thermoplasma acidophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kcat is stable over pH-range 6.0-8.0	Thermoplasma acidophilum
additional information	-	additional information	kinetics	Sus scrofa
additional information	-	additional information	kinetics	Thermoplasma acidophilum
additional information	-	additional information	kcat with citrate and CoA	Sus scrofa
additional information	-	additional information	kcat with citrate and CoA	Thermoplasma acidophilum
9.17	-	acetyl-CoA	recombinant wild-type, pH 8.0	Thermoplasma acidophilum
9.17	-	citrate	recombinant enzyme, pH 8.0	Thermoplasma acidophilum
117	-	citrate	-	Sus scrofa
167	-	acetyl-CoA	pH 7.5	Sus scrofa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	pI: 8.9	Thermoplasma acidophilum

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Release 2023.1 (January 2023)