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Literature summary for 2.3.3.16 extracted from

  • Pettersson, H.; Olsson, P.; Bulow, L.; Pettersson, G.
    Kinetics of the coupled reaction catalyzed by a fusion protein of yeast mitochondrial malate dehydrogenase and citrate synthase (2000), Eur. J. Biochem., 267, 5041-5046.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
cloning of His-tagged fusion protein of citrate synthase and mitochondrial malate dehydrogenase, expression in Escherichia coli Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information no channeling of oxaloacetate between malate dehydrogenase and citrate synthase in a recombinant fusion protein using a coupled assay Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 recombinant fusion protein of yeast citrate synthase and mitochondrial malate dehydrogenase
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged fusion protein of citrate synthase and mitochondrial malate dehydrogenase from E. coli Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + H2O + oxaloacetate = citrate + CoA free diffusion mechanism, no channeling of oxaloacetate from malate dehydrogenase Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
213
-
 purified fusion protein of citrate synthase and mitochondrial malate dehydrogenase, coupled assay Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O no channeling of oxaloacetate between malate dehydrogenase and citrate synthase in a recombinant fusion protein using a coupled assay Saccharomyces cerevisiae citrate + CoA
-
 ir