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Literature summary for 2.3.3.16 extracted from

  • Asai, T.; Takahashi, R.; Fujiola, T.; Tokuyama, T.
    Purification and properties of a citrate synthase from Nitrosomonas sp. TK794 and a comparison with the enzymes of another nitrifying bacteria (1995), J. Ferment. Bioeng., 79, 67-69.
No PubMed abstract available
Search for more literature for 2.3.3.16

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate competitive against oxaloacetate; no inhibition Nitrosomonas sp.
ATP competitive against acetyl-CoA; no inhibition Nitrosomonas sp.
HgCl2
-
 Nitrosomonas sp.
additional information not affected by EDTA Nitrosomonas sp.
N-ethylmaleimide strong Nitrosomonas sp.
p-chloromercuribenzoate strong Nitrosomonas sp.
SDS strong inhibition Nitrosomonas sp.
Zn2+
-
 Nitrosomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 oxaloacetate
-
 Nitrosomonas sp.
0.247
-
 acetyl-CoA
-
 Nitrosomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
KCl at 0.1-100 mM Nitrosomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44700
-
 6 * 44700, SDS-PAGE Nitrosomonas sp.
287000
-
 gel filtration Nitrosomonas sp.

Organism

Organism UniProt Comment Textmining
Nitrosomonas sp.
-
-
-
Nitrosomonas sp. TK794
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nitrosomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
288
-
 purified enzyme Nitrosomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
 Nitrosomonas sp. citrate + CoA
-
 ?
acetyl-CoA + oxaloacetate + H2O
-
 Nitrosomonas sp. TK794 citrate + CoA
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 44700, SDS-PAGE Nitrosomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Nitrosomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable Nitrosomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 5.0 Nitrosomonas sp.
7.5 8
-
 Nitrosomonas sp.
8.5
-
-
 Nitrosomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8.5
-
 Nitrosomonas sp.