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Literature summary for 2.3.3.16 extracted from

  • Pereira, D.S.; Donald, L.J.; Hesfield, D.J.; Duckworth, H.W.
    Active site mutants of Escherichia coli citrate synthase (1994), J. Biol. Chem., 269, 412-417.
    View publication on PubMed
Search for more literature for 2.3.3.16

Protein Variants

Protein Variants Comment Organism
D362A acetyl-CoA binding site mutant, reduced turnover, increased Ki for oxaloacetate and 2-oxoglutarate Escherichia coli
F383A acetyl-CoA binding site mutant, reduced turnover Escherichia coli
H229Q active site mutant, reduced turnover, increased Ki for 2-oxoglutarate Escherichia coli
H264A acetyl-CoA binding site mutant, reduced turnover, increased Ki for oxaloacetate and 2-oxoglutarate Escherichia coli
H305A active site mutant, reduced turnover Escherichia coli
additional information effect of active-site mutantions on substrate binding Escherichia coli
R314L active site mutant, reduced turnover Escherichia coli
R387L active site mutant, reduced turnover Escherichia coli
R407L active site mutant, reduced turnover, increased Ki for oxaloacetate and 2-oxoglutarate Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate wild-type and mutants Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information mutants, with and without KCl, overview Escherichia coli
0.026
-
 oxaloacetate wild-type Escherichia coli
0.12
-
 acetyl-CoA wild-type Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
KCl mutants, overview Escherichia coli
KCl 39fold activation at saturating concentration, wild-type Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
 Escherichia coli citrate + CoA
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information mutants, overview Escherichia coli
0.76
-
 2-oxoglutarate wild-type Escherichia coli