Any feedback?
Literature summary for 2.3.3.16 extracted from
- Mitochondrial citrate synthase from potato: predominant expression in mature leaves and young flower buds (1995), Planta, 196, 756-764.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional expression in citrate synthase deficient Escherichia coli mutant strain K214 under control of lacZ promotor, DNA and amino acid sequence analysis | Solanum tuberosum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Solanum tuberosum | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid sequence comparison | Solanum tuberosum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | Solanum tuberosum | regulation | citrate + CoA | - |
? |  |
| acetyl-CoA + oxaloacetate + H2O | Solanum tuberosum | entry step to tricarboxylic acid cycle | citrate + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | - |
cv. Desiree | - |
| Solanum tuberosum | - |
mitochondrial isozyme | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower bud | - |
Solanum tuberosum | - |
| leaf | 3fold higher in fully mature leaves than immature, declines in senescent leaves, expression and activity under developmental control | Solanum tuberosum | - |
| stolon | - |
Solanum tuberosum | - |
| tuber | - |
Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
northern blot analysis, activity in tissues | Solanum tuberosum |
| 0.02 | - |
recombinant enzyme in crude E. coli enzyme extract | Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + oxaloacetate + H2O | - |
Solanum tuberosum | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | regulation | Solanum tuberosum | citrate + CoA | - |
? | |
| acetyl-CoA + oxaloacetate + H2O | entry step to tricarboxylic acid cycle | Solanum tuberosum | citrate + CoA | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
enzyme assay at | Solanum tuberosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
enzyme assay at | Solanum tuberosum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
