Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | competitive against acetyl-CoA | Saccharolobus solfataricus | |
NADH | competitive against acetyl-CoA | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
acetyl-CoA | - |
Saccharolobus solfataricus | |
0.02 | - |
oxaloacetate | - |
Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Saccharolobus solfataricus |
80000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18 | - |
purified enzyme | Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
-20°C, Tris-HCl buffer, pH 8.0, 1 year | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | - |
Saccharolobus solfataricus | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
100% stable, 10 min | Saccharolobus solfataricus |
95 | - |
90% loss of activity after 10 min | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
ATP | - |
Saccharolobus solfataricus | |
3 | 4 | NADH | - |
Saccharolobus solfataricus | |
3.5 | - |
NADH | - |
Saccharolobus solfataricus |