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Literature summary for 2.3.3.16 extracted from

  • Robinson, M.S.; Danson, M.J.; Weitzman, P.D.
    Citrate synthase from a gram-positive bacterium (1983), Biochem. J., 213, 53-59.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
very instable without glycerol Priestia megaterium

Inhibitors

Inhibitors Comment Organism Structure
ADP 10 mM, 64% inhibition; 64% inhibition at 10 mM Priestia megaterium
AMP 10 mM, 36% inhibition; 36% inhibition at 10 mM Priestia megaterium
ATP 10 mM, 79% inhibition; 79% inhibition at 10 mM Priestia megaterium
NAD+ 10 mM, 9% inhibition Priestia megaterium
NADH 10 mM, 31% inhibition; 1-10 mM; non-specific, 31% inhibition at 10 mM Priestia megaterium
NADP+ 10 mM, 32% inhibition Priestia megaterium
NADPH 10 mM, 57% inhibition; 57% inhibition at 10 mM Priestia megaterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
oxaloacetate
-
Priestia megaterium
0.076
-
acetyl-CoA
-
Priestia megaterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40300
-
2 * 40300, SDS-PAGE, gel filtration in guanidine-HCl, amino acid composition Priestia megaterium
40300
-
2 * 40300, SDS-PAGE, gel filtration in guanidinium chloride Priestia megaterium
84000
-
gel filtration Priestia megaterium
84000
-
Stokes radius, sedimentation coefficient Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-
Priestia megaterium D101
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Priestia megaterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.6
-
-
Priestia megaterium
21.6
-
purified enzyme Priestia megaterium

Storage Stability

Storage Stability Organism
4°C, pure citrate synthase is completely stable for at least 6 months Priestia megaterium
4°C, Tris-HCl buffer, pH 8.0, 20% v/v glycerol and 0.1 M KCl, at least 6 months Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + oxaloacetate + H2O
-
Priestia megaterium citrate + CoA
-
?
acetyl-CoA + oxaloacetate + H2O
-
Priestia megaterium D101 citrate + CoA
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40300, SDS-PAGE, gel filtration in guanidine-HCl, amino acid composition Priestia megaterium
dimer 2 * 40300, SDS-PAGE, gel filtration in guanidinium chloride Priestia megaterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
enzyme assay at Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
enzyme assay at Priestia megaterium