Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-ketoglutarate | - |
Acinetobacter calcoaceticus subsp. anitratus | |
AMP | slight activation | Acinetobacter calcoaceticus subsp. anitratus |
General Stability | Organism |
---|---|
very labile, glycerol, 20% v/v or more stabilizes | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | no inhibition | Acinetobacter calcoaceticus subsp. anitratus | |
acetyl-CoA | no inhibition | Azotobacter vinelandii | |
acetyl-CoA | substrate inhibition at high concentration | Bacillus subtilis | |
ATP | possibly involved in enzyme regulation | Acinetobacter calcoaceticus subsp. anitratus | |
ATP | possibly involved in enzyme regulation | Azotobacter vinelandii | |
ATP | possibly involved in enzyme regulation | Bacillus subtilis | |
citrate | competitive with acetyl-CoA, noncompetitive with oxaloacetate | Acinetobacter calcoaceticus subsp. anitratus | |
citrate | competitive with acetyl-CoA, noncompetitive with oxaloacetate | Azotobacter vinelandii | |
citrate | competitive with acetyl-CoA, noncompetitive with oxaloacetate | Bacillus subtilis | |
CoA | competitive with acetyl-CoA, noncompetitive with oxaloacetate | Acinetobacter calcoaceticus subsp. anitratus | |
CoA | competitive with acetyl-CoA, noncompetitive with oxaloacetate | Azotobacter vinelandii | |
CoA | competitive with acetyl-CoA, noncompetitive with oxaloacetate | Bacillus subtilis | |
NADH | 12% inhibition at 1 mM | Acinetobacter calcoaceticus subsp. anitratus | |
NADH | - |
Azotobacter vinelandii | |
NADPH | 29% inhibition at 0.25 mM | Acinetobacter calcoaceticus subsp. anitratus | |
oxaloacetate | no inhibition | Acinetobacter calcoaceticus subsp. anitratus | |
oxaloacetate | no inhibition | Azotobacter vinelandii | |
oxaloacetate | substrate inhibition | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
oxaloacetate | - |
Azotobacter vinelandii | |
0.08 | - |
acetyl-CoA | - |
Azotobacter vinelandii | |
0.1 | - |
acetyl-CoA | - |
Acinetobacter calcoaceticus subsp. anitratus | |
0.6 | - |
acetyl-CoA | - |
Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Acinetobacter calcoaceticus subsp. anitratus | |
KCl | - |
Azotobacter vinelandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58500 | - |
4 * 58500, sedimentation equilibrium in guanidine-HCl and dithiothreitol | Azotobacter vinelandii |
60000 | 95000 | gel filtration, sedimentation equilibrium centrifugation | Bacillus subtilis |
69000 | - |
4 * 69000, sedimentation equilibrium in guanidine-HCl and dithiothreitol | Acinetobacter calcoaceticus subsp. anitratus |
242000 | - |
sedimentation equilibrium centrifugation | Acinetobacter calcoaceticus subsp. anitratus |
250000 | - |
sedimentation equilibrium centrifugation | Azotobacter vinelandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | Bacillus subtilis | entry step to tricarboxylic acid cycle | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | Acinetobacter calcoaceticus subsp. anitratus | entry step to tricarboxylic acid cycle | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | Azotobacter vinelandii | entry step to tricarboxylic acid cycle | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | Bacillus subtilis HS2A2 | entry step to tricarboxylic acid cycle | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | Azotobacter vinelandii OP | entry step to tricarboxylic acid cycle | citrate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus subsp. anitratus | - |
- |
- |
Azotobacter vinelandii | - |
- |
- |
Azotobacter vinelandii OP | - |
- |
- |
Bacillus subtilis | - |
- |
- |
Bacillus subtilis HS2A2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter calcoaceticus subsp. anitratus |
- |
Azotobacter vinelandii |
partial | Bacillus subtilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + H2O + oxaloacetate = citrate + CoA | random sequential reaction order mechanism | Bacillus subtilis | |
acetyl-CoA + H2O + oxaloacetate = citrate + CoA | random sequential reaction order mechanism | Acinetobacter calcoaceticus subsp. anitratus | |
acetyl-CoA + H2O + oxaloacetate = citrate + CoA | random sequential reaction order mechanism | Azotobacter vinelandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.6 | - |
partially purified enzyme | Bacillus subtilis |
52.3 | - |
purified enzyme | Azotobacter vinelandii |
235 | - |
purified enzyme | Acinetobacter calcoaceticus subsp. anitratus |
523 | - |
- |
Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | - |
Bacillus subtilis | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | - |
Acinetobacter calcoaceticus subsp. anitratus | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | - |
Azotobacter vinelandii | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | entry step to tricarboxylic acid cycle | Bacillus subtilis | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | entry step to tricarboxylic acid cycle | Acinetobacter calcoaceticus subsp. anitratus | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | entry step to tricarboxylic acid cycle | Azotobacter vinelandii | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | - |
Bacillus subtilis HS2A2 | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | entry step to tricarboxylic acid cycle | Bacillus subtilis HS2A2 | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | - |
Azotobacter vinelandii OP | citrate + CoA | - |
? | |
acetyl-CoA + oxaloacetate + H2O | entry step to tricarboxylic acid cycle | Azotobacter vinelandii OP | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 58500, sedimentation equilibrium in guanidine-HCl and dithiothreitol | Azotobacter vinelandii |
tetramer | 4 * 69000, sedimentation equilibrium in guanidine-HCl and dithiothreitol | Acinetobacter calcoaceticus subsp. anitratus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
enzyme assay at | Bacillus subtilis |
30 | - |
enzyme assay at | Acinetobacter calcoaceticus subsp. anitratus |
30 | - |
enzyme assay at | Azotobacter vinelandii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
942 | - |
citrate | - |
Acinetobacter calcoaceticus subsp. anitratus | |
2100 | - |
citrate | - |
Azotobacter vinelandii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.75 | - |
ATP | versus acetyl-CoA | Azotobacter vinelandii | |
1 | - |
ATP | - |
Bacillus subtilis |