Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Rosetta 2 cells | Aspergillus fumigatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-methylcitrate | 89.6% residual activity at 5 mM | Aspergillus fumigatus | |
Ca2+ | 96.3% residual activity at 1 mM | Aspergillus fumigatus | |
citrate | 66.1% residual activity at 10 mM | Aspergillus fumigatus | |
Cu2+ | 0.7% residual activity at 0.025 mM | Aspergillus fumigatus | |
dipyridyl | 95% residual activity at 0.1 mM | Aspergillus fumigatus | |
EDTA | 13.4% residual activity at 5 mM | Aspergillus fumigatus | |
K+ | 91.7% residual activity at 0.1 mM | Aspergillus fumigatus | |
L-lysine | 24.4% residual activity at 50 mM | Aspergillus fumigatus | |
Na2SO4 | 90.9% residual activity at 0.2 mM | Aspergillus fumigatus | |
Zn2+ | 0.8% residual activity at 0.1 mM | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
acetyl-CoA | in 200 mM HEPES, pH 7.5, at 22°C | Aspergillus fumigatus | |
0.261 | - |
2-oxoglutarate | in 200 mM HEPES, pH 7.5, at 22°C | Aspergillus fumigatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme activity is independent of any metal ions | Aspergillus fumigatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50600 | - |
x * 50600, deduced from amino acid sequence | Aspergillus fumigatus |
51000 | - |
x * 51000, SDS-PAGE | Aspergillus fumigatus |
66000 | - |
different bands are detected at 66 kDa (monomer), 148 kDa (dimer), 226 kDa (trimer), and 280 kDa (tetramer), and the dimeric and tetrameric compositions are most abundant, gel filtration | Aspergillus fumigatus |
148000 | - |
different bands are detected at 66 kDa (monomer), 148 kDa (dimer), 226 kDa (trimer), and 280 kDa (tetramer), and the dimeric and tetrameric compositions are most abundant, gel filtration | Aspergillus fumigatus |
226000 | - |
different bands are detected at 66 kDa (monomer), 148 kDa (dimer), 226 kDa (trimer), and 280 kDa (tetramer), and the dimeric and tetrameric compositions are most abundant, gel filtration | Aspergillus fumigatus |
280000 | - |
different bands are detected at 66 kDa (monomer), 148 kDa (dimer), 226 kDa (trimer), and 280 kDa (tetramer), and the dimeric and tetrameric compositions are most abundant, gel filtration | Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | C7C437 | formerly Aspergillus fumigatus | - |
Aspergillus fumigatus CBS 144.89 | C7C437 | formerly Aspergillus fumigatus | - |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Aspergillus fumigatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.8 | - |
purified enzyme, in 200 mM HEPES, pH 7.5, at 22°C | Aspergillus fumigatus |
Storage Stability | Organism |
---|---|
-20°C or -80°C, HEPES buffer with guanidine hydrochloride, alpha-cyclodextrin, ammonium sulfate, KCl and 20% (v/v) glycerol, several months, no significant loss of activity | Aspergillus fumigatus |
4°C, buffer without stabilizing components, 72 h, complete loss of activity | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + 2-oxoglutarate | specific substrates | Aspergillus fumigatus | 2-hydroxybutane-1,2,4-tricarboxylate + CoA | - |
? | |
acetyl-CoA + H2O + 2-oxoglutarate | specific substrates | Aspergillus fumigatus CBS 144.89 | 2-hydroxybutane-1,2,4-tricarboxylate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51000, SDS-PAGE | Aspergillus fumigatus |
? | x * 50600, deduced from amino acid sequence | Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
HcsA | - |
Aspergillus fumigatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 52 | - |
Aspergillus fumigatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
60 | the enzyme retains 100% activity after more than 8 h at 0-37°C, 70% residual activity after 270 min at 42°C, and 50% residual activity after 2 min at 60°C | Aspergillus fumigatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.68 | - |
acetyl-CoA | in 200 mM HEPES, pH 7.5, at 22°C | Aspergillus fumigatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.2 | - |
Aspergillus fumigatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | the enzyme retains 70% residual activity after 6 h at pH 4.0, 100% activity after 6 h at pH 5.0-6.0, 80% residual activity after 6.5 h at pH 7.0-9.0, and 50% residual activity after 50 min at pH 11.0 | Aspergillus fumigatus |
Organism | Comment | Expression |
---|---|---|
Aspergillus fumigatus | gene expression increases under conditions of lysine supplementation | up |
General Information | Comment | Organism |
---|---|---|
malfunction | an hcsA deletion mutant is lysine auxotrophic and unable to germinate on unhydrolyzed proteins given as a sole nutrient source. The mutant grows on blood and serum, indicating the existence of high-affinity lysine uptake systems. The virulence of the mutant is strongly attenuated in murine models of bronchopulmonary aspergillosis. The mutant retains full virulence when injected intravenously | Aspergillus fumigatus |