Cloned (Comment) | Organism |
---|---|
expressed at high levels in Escherichia coli | Azotobacter vinelandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
acetyl-CoA | reaction with 2-oxoglutarate | Azotobacter vinelandii | |
1.24 | - |
2-oxoadipate | - |
Azotobacter vinelandii | |
2.24 | - |
2-oxoglutarate | - |
Azotobacter vinelandii | |
2.28 | - |
oxaloacetate | - |
Azotobacter vinelandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89000 | - |
gel filtration | Azotobacter vinelandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Oxidation Stability | Organism |
---|---|
loss of approximately 50% of its activity within 2 h upon exposure to air | Azotobacter vinelandii |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Azotobacter vinelandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.657 | - |
- |
Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + 2-oxoadipate | - |
Azotobacter vinelandii | 2-hydroxyhexane-1,2,6-tricarboxylate | - |
? | |
acetyl-CoA + H2O + 2-oxoglutarate | - |
Azotobacter vinelandii | 2-hydroxybutane-1,2,4-tricarboxylate + CoA | - |
? | |
acetyl-CoA + H2O + oxaloacetate | - |
Azotobacter vinelandii | 2-hydroxypropane-1,2,3-tricarboxylate + CoA | - |
? |