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Literature summary for 2.3.3.14 extracted from

  • Takenouchi, E.; Tanaka, H.; Soda, K.
    Homocitrate synthase of S-(beta-aminoethyl)-L-cysteine resistant mutant of Candida pelliculosa (1981), J. Ferment. Technol., 59, 429-433.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
DL-Homolysine inhibition of enzyme from wild-type strain, enzyme from S-(beta-aminoethyl)-L-cysteine resistant mutant strain is not inhibited Wickerhamomyces anomalus
Hg2+
-
Wickerhamomyces anomalus
L-Lys 0.02 mM required for half-maximal inhibition of the enzyme from wild-type strain, enzyme from S-(beta-aminoethyl)-L-cysteine resistant mutant strain is not inhibited Wickerhamomyces anomalus
NEM 0.1 mM, 90% inhibition Wickerhamomyces anomalus
PCMB 0.1 mM Wickerhamomyces anomalus
S-(beta-aminoethyl)-L-cysteine 1.1 mM required for half-maximal inhibition of enzyme from wild-type strain, enzyme from S-(beta-aminoethyl)-L-cysteine resistant mutant strain is not inhibited Wickerhamomyces anomalus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
acetyl-CoA enzyme from S-(beta-aminoethyl)-L-cysteine resistant mutant strain Wickerhamomyces anomalus
0.2
-
acetyl-CoA enzyme from wild-type strain Wickerhamomyces anomalus
10
-
2-oxoglutarate enzyme from S-(beta-aminoethyl)-L-cysteine resistant mutant strain Wickerhamomyces anomalus
11.8
-
2-oxoglutarate enzyme from wild-type strain Wickerhamomyces anomalus

Organism

Organism UniProt Comment Textmining
Wickerhamomyces anomalus
-
-
-

Storage Stability

Storage Stability Organism
0-5°C, 0.1 M potassium phosphate buffer, pH 7.8, containing 20% glycerol and 1 mM dithiothreitol, about 50% loss of activity after 2 weeks Wickerhamomyces anomalus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + 2-oxoglutarate
-
Wickerhamomyces anomalus 2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
wild-type strain Wickerhamomyces anomalus
43
-
enzyme from S-(beta-aminoethyl)-L-cysteine resistant mutant strain Wickerhamomyces anomalus