Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-Homolysine | inhibition of enzyme from wild-type strain, enzyme from S-(beta-aminoethyl)-L-cysteine resistant mutant strain is not inhibited | Wickerhamomyces anomalus | |
Hg2+ | - |
Wickerhamomyces anomalus | |
L-Lys | 0.02 mM required for half-maximal inhibition of the enzyme from wild-type strain, enzyme from S-(beta-aminoethyl)-L-cysteine resistant mutant strain is not inhibited | Wickerhamomyces anomalus | |
NEM | 0.1 mM, 90% inhibition | Wickerhamomyces anomalus | |
PCMB | 0.1 mM | Wickerhamomyces anomalus | |
S-(beta-aminoethyl)-L-cysteine | 1.1 mM required for half-maximal inhibition of enzyme from wild-type strain, enzyme from S-(beta-aminoethyl)-L-cysteine resistant mutant strain is not inhibited | Wickerhamomyces anomalus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
acetyl-CoA | enzyme from S-(beta-aminoethyl)-L-cysteine resistant mutant strain | Wickerhamomyces anomalus | |
0.2 | - |
acetyl-CoA | enzyme from wild-type strain | Wickerhamomyces anomalus | |
10 | - |
2-oxoglutarate | enzyme from S-(beta-aminoethyl)-L-cysteine resistant mutant strain | Wickerhamomyces anomalus | |
11.8 | - |
2-oxoglutarate | enzyme from wild-type strain | Wickerhamomyces anomalus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Wickerhamomyces anomalus | - |
- |
- |
Storage Stability | Organism |
---|---|
0-5°C, 0.1 M potassium phosphate buffer, pH 7.8, containing 20% glycerol and 1 mM dithiothreitol, about 50% loss of activity after 2 weeks | Wickerhamomyces anomalus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + 2-oxoglutarate | - |
Wickerhamomyces anomalus | 2-hydroxybutane-1,2,4-tricarboxylate + CoA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
wild-type strain | Wickerhamomyces anomalus |
43 | - |
enzyme from S-(beta-aminoethyl)-L-cysteine resistant mutant strain | Wickerhamomyces anomalus |