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Literature summary for 2.3.3.13 extracted from
- Crystallization and preliminary X-ray analysis of alpha-isopropylmalate synthase from Mycobacterium tuberculosis (2004), Acta Crystallogr. Sect. D, 60, 1167-1169.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | potential target for drugs against microbial pathogens due to the absence of this enzyme in mammals | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as His-tag fusion protein in Escherichia coli BL21(DE3) | Mycobacterium tuberculosis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + alpha-ketoisovalerate + H2O | Mycobacterium tuberculosis | first step of leucine biosynthesis | alpha-isopropylmalate + CoA | - |
? |  |
| acetyl-CoA + alpha-ketoisovalerate + H2O | Mycobacterium tuberculosis H37Rv | first step of leucine biosynthesis | alpha-isopropylmalate + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme using His-tag | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + alpha-ketoisovalerate + H2O | first step of leucine biosynthesis | Mycobacterium tuberculosis | alpha-isopropylmalate + CoA | - |
? | |
| acetyl-CoA + alpha-ketoisovalerate + H2O | reaction | Mycobacterium tuberculosis | alpha-isopropylmalate + CoA | - |
? | |
| acetyl-CoA + alpha-ketoisovalerate + H2O | first step of leucine biosynthesis | Mycobacterium tuberculosis H37Rv | alpha-isopropylmalate + CoA | - |
? | |
| acetyl-CoA + alpha-ketoisovalerate + H2O | reaction | Mycobacterium tuberculosis H37Rv | alpha-isopropylmalate + CoA | - |
? | |
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