Cloned (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Oxo-isohexanoate | competitive | Saccharomyces cerevisiae | |
2-oxopentanoate | competitive | Saccharomyces cerevisiae | |
CoA | Zn2+-dependent reversible inactivation | Saccharomyces cerevisiae | |
leucine | pH: 7.2, complete inhibition, pH: 8.0, 70% inhibition, pH: 8.8, 15% inhibition | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
0.2 | - |
pyruvate | - |
Saccharomyces cerevisiae | |
0.57 | - |
2-oxobutanoate | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | alpha-isopropylmalate synthase Ib | Saccharomyces cerevisiae | 5737 | - |
mitochondrion | alpha-isopropylmalate synthase Ia, MW 68000, is imported into the mitochondrial matrix | Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | enzyme requires the presence of monovalent cations, K+ is most effective | Saccharomyces cerevisiae | |
Zinc | enzyme of strain S288C contains 2 gatoms of zinc per subunit | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68416 | - |
x * 68416, isoenzyme I, calculation from nucleotide sequence | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | Saccharomyces cerevisiae | first enzyme in biosynthesis of L-Leu | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
3 enzyme forms, 1. alpha-isopropylmalate synthase I, isoenzyme encoded by LEU, subform Ia, MW 68000, which is imported into the mitochondrial matrix, and cytoplasmic synthase Ib, 2. alpha-isopropylmalate synthase II, minor enzyme form | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.1 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | i.e. alpha-isopropylmalate | ? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | first enzyme in biosynthesis of L-Leu | Saccharomyces cerevisiae | ? | - |
? | |
2-oxo-butanoate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae | 2-ethyl-2-hydroxysuccinic acid + CoA | - |
? | |
pyruvate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae | 2-hydroxy-2-methylsuccinic acid + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68416, isoenzyme I, calculation from nucleotide sequence | Saccharomyces cerevisiae |
dimer | 2 * 65000-68000 | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8.5 | - |
Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
activity of enzyme drops off rapidly below pH 7.0 | Saccharomyces cerevisiae |
7.2 | 8.2 | activity of enzyme drops off rapidly above pH 9.0 | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
CoA | interacting with the product site | Saccharomyces cerevisiae | |
0.1 | - |
leucine | only isoenzyme I activity | Saccharomyces cerevisiae | |
0.2 | - |
leucine | pH: 7.2, half-maximal inhibition | Saccharomyces cerevisiae | |
1.2 | - |
leucine | only isoenzyme II activity | Saccharomyces cerevisiae |