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Literature summary for 2.3.3.13 extracted from

  • Webster, R.E.; Gross, S.R.
    The alpha-isopropylmalate synthetase of Neurospora crassa. I. The kinetics and end product control of alpha-isopropylmalate synthetase function (1965), Biochemistry, 4, 2309-2318.
No PubMed abstract available
Search for more literature for 2.3.3.13

Inhibitors

Inhibitors Comment Organism Structure
5',5',5'-trifluoroleucine reversed by acetyl-CoA Neurospora crassa
Br- above 0.02 M Neurospora crassa
DL-azaleucine 10 mM, at pH 6.5 Neurospora crassa
DL-fluoroleucine at pH 7.5 Neurospora crassa
F- above 0.02 M Neurospora crassa
L-Phe 10 mM, at pH 6.5 Neurospora crassa
L-Val 10 mM, at pH 6.5 Neurospora crassa
leucine at pH 7.5 Neurospora crassa
Zn2+ 0.1 mM Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 2-oxo-3-methylbutanoate
-
 Neurospora crassa
0.0245
-
 acetyl-CoA pH 7.5 Neurospora crassa
0.028
-
 acetyl-CoA pH 7.0 Neurospora crassa
0.035
-
 acetyl-CoA pH 6.6 Neurospora crassa
2.1
-
 2-oxo-butanoate
-
 Neurospora crassa
5.8
-
 pyruvate
-
 Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
 Neurospora crassa
Li+ stimulates at low concentrations Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxo-3-methylbutanoate + acetyl-CoA + H2O Neurospora crassa first enzyme in biosynthesis of L-Leu ?
-
 ?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Neurospora crassa

Storage Stability

Storage Stability Organism
-20°C, phosphate leucine buffer, little loss of activity Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
 Neurospora crassa 3-hydroxy-4-methyl-3-carboxypentanoate + CoA
-
 ?
2-oxo-3-methylbutanoate + acetyl-CoA + H2O first enzyme in biosynthesis of L-Leu Neurospora crassa ?
-
 ?
2-oxo-butanoate + acetyl-CoA + H2O
-
 Neurospora crassa 2-ethyl-2-hydroxysuccinic acid + CoA
-
 ?
pyruvate + acetyl-CoA + H2O
-
 Neurospora crassa 2-hydroxy-2-methylsuccinic acid + CoA
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 thermal stability at pH 6.5 increases as a function of Leu concentration. The protection afforded by Leu is greatest at pH 6.5, less at pH 7.5, and is entirely lost at pH 8.3 Neurospora crassa
49
-
 7 min, 90% loss of activity in absence of Leu, about 50% loss of activity after 15 min in presence of 0.2 mM Leu Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Neurospora crassa